Emd Millipore Corporation

Millipore Corporation

David Yavorsky

John Paul Amara

Mikhail Kozlov

Matthew T Stone

Joaquin A Umana

William Cataldo

Kwok-Shun Cheng

Nripen Singh

Maybelle Woo

John Boyle

Ven Anantha Raman

Benjamin Cacace

Jonathan Royce

Matthew Alexander Tomasko

A depth filtration device for the clarification of biological fluids including a composite depth filter media having a nonwoven first layer integral with a second layer containing a polyacrylonitrile (PAN) fibers, a filter aid, and a wet-strength resin. The depth filter media exhibits increased binding capacity for soluble impurities such as DNA and host cell proteins from biological/cell culture feedstreams during secondary clarification and low-level impurity clearance of harvested cell culture fluids, such as those used for the manufacture of monoclonal antibodies. The depth filter media additionally exhibits significantly lower flushing requirements, resulting in lower levels of organic, inorganic and bioburden extractables released, high dirt holding capacities and good chemical and/or radiation resistance.

High capacity composite depth filter media with low extractables

Adsorptive media for chromatography, particularly ion-exchange chromatography, derived from a shaped fiber. In certain embodiments, the functionalized shaped fiber presents a fibrillated or ridged structure which greatly increases the surface area of the fibers when compared to ordinary fibers. Also disclosed herein is a method to add surface pendant functional groups that provides cation-exchange or anion-exchange functionality to the high surface area fibers. This pendant functionality is useful for the ion-exchange chromatographic purification of biomolecules, such as monoclonal antibodies (mAbs).

Chromatography media and method

Chromatography media including a high surface area thermoplastic porous nanofiber and an ion-exchange ligand functionality on the surface of the fiber. The porous nanofibers display a convoluted structure that is comprised of discrete bundles of highly entangled nanofibrils that may be fibrillated or ridged. The porous fibers can be prepared through the extraction of a dissolvable mineral or polymeric porogen that is embedded into the fiber during its manufacture in a melt extrusion process.

High surface area fiber media with nano-fibrillated surface features

Adsorbent filter media particularly suited for removal of biological contaminants in process liquids. A porous fixed bed of adsorbent material is formed, using only a granular adsorbent and a water-insoluble thermoplastic binder. The resulting composite filter allows for a higher amount of adsorbent with smaller adsorbent particles than conventional depth filters. Elimination of cellulose fiber, as well as the elimination of the thermoset binder, results in reduced contamination of the process liquid.

Adsorbent filter media for removal of biological contaminants in process liquids

A deep gradient-density filter device capable of effectively filtering a fluid containing a distribution of particles in the range of approximately 25 microns to approximately 0.2 micron at a fluid velocity of at least approximately 100 cm/hr and an initial hydraulic permeability of greater than approximately 10 cm/hr/psi. The deep gradient-density filter device includes several layers of filtration material, each having specific predetermined particle retention properties. In one product embodiment, the deep gradient-density filter device uses 'loose' fibrillated cellulose fiber material as a primary filter element. In a method aspect, the deep gradient-density filter device is used for either the secondary clarification of industrial-scale volumes of cultured or fermented protein-containing biopharmaceutical fluids or the primary clarification of pilot-scale volumes.

Deep gradient-density filter device

Growing community of inventors

Bolton, MA, United States of America

David Yavorsky