Nanogen, Inc.

Gamida for Life B.v.

David Willoughby

Jing Cheng

Michael James Heller

Lei Wu

James P O'Connell

Jonathan M Diver

Dan Smolko

Shila Jalali

Ed Sheldon

Edward L Sheldon

We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a 'laboratory-on-a-chip' system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.

Integrated portable biological detection system

We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a &ldquo;laboratory-on-a-chip&rdquo; system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.

Growing community of inventors

San Diego, CA, United States of America

David Willoughby