Miltenyi Biotec, Gmbh

Miltenyi Biotech Gmbh

David William Buck

Andrzej Dzionek

Juergen Schmitz

This invention provides a method of detecting BDCA-2 protein in a biological sample. The sample is combined with an antibody or antigen binding fragment under conditions where the antibody or fragment will bind to BDCA-2 protein in the sample to form a complex, which is detected by formation of complex with the antibody. BDCA-2 protein may be displayed on the surface of dendritic cells. Binding of the antibody may result in down-regulation of type 1 interferon production, down-regulation of Th1 immune responses, induction of intracellular Camobilization, and/or polarization of an immune response towards Th2.

Ligating BDCA-2 protein with BDCA-2 specific antibody for detecting or modulating dendritic cells

The application describes, inter alia, a method for isolating or enriching for dendritic cells from a mixture of human cells involving contacting cells in the mixture with an antibody that binds BDCA-4, or antigen-binding fragment thereof, under conditions in which the antibody or antigen-binding fragment binds BDCA-4+ dendritic cells, and separating dendritic cells bound by the antibody or antigen-binding fragment from other cells in the mixture.

Method for isolating BDCA-4+ dendritic cells

The invention provides antigen-binding fragments specific for dendritic cells and effective in treatment and/or diagnosing a variety of disorders. Methods of use are also provided as are methods for screening for additional such antigen-binding fragments and the products obtained thereby.

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Mayfield, United Kingdom

David William Buck