Nitto-denko Corporation

Arcturus Therapeutics, Inc.

David Webb

Priya Prakash Karmali

Victor Knopov

Richard P Witte

Robin Lee

Joseph E Payne

Padmanabh Chivukula

Violetta Akopian

Priya Karmali

What is described is a method for preparing a liposome that efficiently encapsulates a negatively charged therapeutic polymer, e.g., siRNA. The process involves preparing a lipid mixture comprising a cationic lipid in a water miscible organic solvent, such as ethanol, at a concentration of 2.3 mg/ml, and adding this solution to the polymer dissolved in water to a final concentration of 35% ethanol in water. The final charge ratio of drug:lipid is 1:2.5. The resulting nanoparticles have a mean size of 50 to 150 nm.

Method of producing lipid nanoparticles for drug delivery

What is described is a lipid particle comprising the compound of formula I wherein

Lipid particles with asymmetric cationic lipids for RNA delivery

What is described is a compound of formula I wherein

Asymmetric ionizable cationic lipid for RNA delivery

What is described is a process of forming lipid-nucleic acid nanoparticles simply and reproducibly under aseptic conditions comprising single use components.

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Oceanside, CA, United States of America

David Webb