Promega Corporation

Panvera LLC

David V Thompson

Thomas J Burke

Thomas R Van Oosbree

Martin Ken Lewis

Peter M Shaw

Randall E Bolger

William J Checovich

Gregory S Beckler

Sandra L Spurgeon

John F Van Herwynen

Site-directed in vitro mutagenesis is obtained utilizing a specially-engineered plasmid vector. The vector is engineered to contain an inactivated genetic marker which marker is capable of being reverted to functional expression. Additionally, the vector contains a second genetic marker, a polylinker region and an f1 replication origin.

Vector for in vitro mutagenesis and use thereof

A storable biotransformation mixture that can oxidize a drug, comprising a cytochrome P450 enzyme, an electron donor; and, a buffer solution. In a preferred embodiment the mixture also contains liposomes, cytochrome b.sub.5 and NADPH-P450 reductase as the electron donor. The mixture is storable at a temperature less than 8.degree. C. to for at least two weeks and is able to oxidize a drug after storage.

Compositions for cytochrome P450 biotransformation reactions

The present invention is based on the degradation of a fluorescence labeled nucleic acid such as DNA (F-DNA), RNA (F-RNA) or DNA/RNA substrate. The label may be covalently attached to the relatively high volume substrate at one position or many positions. The nucleic acid is then put in contact with another substance and activity is detected by Fluorescence Polarization.

Method and kit for detecting nucleic acid cleavage utilizing a

A method for coupling transcription and translation from DNA using a solution comprising a eukaryotic cell-free extract comprising adding a sufficient amount of a magnesium compound to the extract to raise the magnesium concentration to a level where RNA is transcribed from DNA and RNA translates into protein.

Coupled transcription and translation in eukaryotic cell-free extract

The present invention is directed to a modified Taq DNA polymerase and methods for its use.

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Monona, WI, United States of America

David V Thompson