Monsanto Technology LLC

David V Butruille

John Tamulonis

Sam R Eathington

Kevin Cook

Hongwu Jia

Travis J Frey

Michael R Kerns

Kevin Lee Deppermann

Michael W Petersen

Bruce J Schnicker

Heather M Forbes

Stanton B Dotson

John R LeDeaux

Gilberto Pozar

James Narvel

Vergel C Concibido

George James Baley

John W Pitkin

Chongqing Xie

Samuel R Eathington

Michael D Haverdink

Warren M Kruger

Gregory J Holland

Scott Walker

Laron L Peters

Jason Kendrick Bull

Anju Gupta

Marlin Edwards

Kunsheng Wu

Richard Johnson

Wayne M Kennard

Jennifer Rinehart

Edward James Cargill

Allen N Ondes

David W Finley

William M Fischer

John M Jensen

Michael A Hall

Charles R Dietrich

Fenggao Dong

Ty Jason Barten

Lex Evan Flagel

Jonathan C Lamb

Robert Stefan Reiter

Paul McLaird

Terri B Hinchey

Ruth A Wagner

Jose Rafael Gomez

Manuel Oyervides Garcia

G Richard Johnson

Yang Ju Im

Fengxing Du

Christopher T Bauer

Daniel Challis

Brian W Gardunia

Piyaraj Nan Newton

Pierre Sehabiague

Shengqiang Zhong

Qiang Zhang

Heather Forbes

A method is provided for use in analyzing seeds, in a population of seeds, for one or more desired characteristic. The method includes removing tissue from individual seeds in a population of seeds using an automated seed sampler, while preserving germination viability of the seeds, and analyzing the tissue for the presence or absence of one or more desired characteristic. The method then includes, based on the analysis of the tissue, quantifying the one or more desired characteristic for each of the individual seeds that possess the one or more desired characteristic.

Methods of seed breeding using high throughput nondestructive seed sampling

Exemplary systems and methods for selecting from population of candidate edits and predicting an aggregate effect of the candidate edits are disclosed. One exemplary method includes identifying a population of candidate edits to a genomic sequence of said organism and ranking each of the candidate edits based on a predicted ability of each candidate edit to affect a trait of interest in said organism. The exemplary method further includes selecting one or more of the candidate edits based on the ranking and predicting, by the computing device, an aggregate effect of the selected one or more of the candidate edits for the trait of interest when expressed by a specimen of the organism having a genomic sequence and edited according to the selected one or more of the candidate edits, as compared to an unedited specimen of the organism.

Systems and methods for use in identifying multiple genome edits and predicting the aggregate effects of the identified genome edits

Methods for providing compositions to corn fields prior to harvesting are provided herein. These methods provide an extended time period for the use of lower height or standard height farm equipment in-season in corn fields, while reducing the risk of damage to the corn plants. These methods also allow for late season access with lower height or standard height farm equipment, while reducing the risk of damage to the corn plants.

Management of corn through semi-dwarf systems

The invention provides novel methods to genotype haploid embryos using molecular assays. For example, quantitative methods for genotyping are provided. The methods provided also include providing a plurality of haploid kernels, determining the genotype of the haploid embryo of said kernels by distinguishing its genotype from the endosperm genotype, selecting a kernel having a desired genotype and producing doubled haploid plant from the selected kernel.

Methods for genotyping haploid embryos

A method is provided for use in a plant breeding program to generate a population having one or more desired traits. The method includes removing a tissue sample from each of one or more seeds using an automated seed sampler while preserving germination viability of the sampled seeds; analyzing the tissue samples for the presence or absence of a genetic sequence associated with at least one desired trait; based on the analysis of the tissue samples, cultivating plants from the sampled seeds that either possess or lack the genetic sequence associated with the at least one desired trait; creating a parental cross using at least one of the cultivated plants; and selecting seeds from the parental cross and cultivating offspring of the parental cross using the selected seeds.

The present invention relates to the field of plant breeding. More specifically, the present invention includes a method of using haploid plants for genetic mapping of traits of interest such as disease resistance. Further, the invention includes a method for breeding corn plants containing quantitative trait loci (QTL) that are associated with resistance to Goss' Wilt, a bacterial disease associated withspp.

Methods and compositions for Goss' Wilt resistance in corn

The present invention relates to the field of plant breeding. More specifically, the present invention includes a method of using haploid plants for genetic mapping of traits of interest such as disease resistance. Further, the invention includes a method for breeding corn plants containing quantitative trait loci (QTL) that are associated with resistance to Gray Leaf Spot, a fungal disease associated withspp.

Methods and compositions for gray leaf spot resistance in corn

A seed sampling system is provided having an automated seed loading assembly including a seed bin and being operable to singulate seeds from a plurality of seeds within the seed bin. The system also includes an automated seed sampling assembly operable to remove tissue samples from the singulated seeds, and an automated seed transport assembly operable to transfer the singulated seeds from the seed loading assembly to the seed sampling assembly. The seed transport assembly includes multiple retention members. Each of the retention members is movable relative to the seed loading assembly and to the seed sampling assembly. The seed transport assembly is operable to position one of the multiple retention members adjacent to the seed loading assembly for engaging one of the singulated seeds, while positioning another of the retention members adjacent to the seed sampling assembly for presenting another of the singulated seeds to the seed sampling assembly.

Automated systems for removing tissue samples from seeds, and related methods

The present invention is in the field of plant breeding and genetics, particularly as it pertains to the genus,. More specifically, the invention relates to a method for screening soybean plants containing one or more quantitative trait loci for disease resistance, species ofhaving such loci and methods for breeding for and screening ofwith such loci. The invention further relates to the use of exotic germplasm in a breeding program.

Method to identify disease resistant quantitative trait loci in soybean and compositions thereof

The present invention relates to breeding methods to enhance the germplasm of a plant. The methods describe the identification and accumulation of preferred haplotype genomic regions in the germplasm of breeding populations of maize () and soybean (). The invention also relates to maize and soybean plants comprising preferred haplotypes.

Compositions and methods of plant breeding using high density marker information

The present invention provides breeding methods and compositions to enhance the germplasm of a plant. The methods describe the identification and accumulation of transgenes and favorable haplotype genomic regions in the germplasm of a breeding population of crop plants.

Methods and compositions to enhance plant breeding

The present invention is in the field of plant breeding and disease resistance. More specifically, the invention includes a method for breeding corn plants containing quantitative trait loci that are associated with resistance to gray leaf spot, a fungal disease associated withspp. The invention further includes germplasm and the use of germplasm containing quantitative trait loci (QTL) conferring disease resistance for introgression into elite germplasm in a breeding program for resistance to gray leaf spot.

Resistance to gray leaf spot in maize

Growing community of inventors

Huxley, IA, United States of America

David V Butruille