Boehringer Mannheim Gmbh

Hapak Enterprises

David Tabb

Myron C Rapkin

Eric R Diebold

Arthur M Usmani

Jim Kennamer

Roger Bontrager

Diane Storhoff

Diana F Storhoff

The invention involves apparatus, reagents, and methods for determining a disinfecting effective amount of hypochlorite in a sample. These involve using enough neutralizing agent sufficient to neutralize up to, but no more, than 0.525%, or 5250 parts per million, of hypochlorite in a sample. If more than this amount is present in the sample, then an indicator system reacts with it to provide a detectable signal, such as color. If the hypochlorite is present at less than 0.525%, no signal forms. One can determine this in two minutes or less.

Apparatus and methods useful in determining disinfectant effective

A stable reagent for assaying an analyte from a fluid sample which includes an enzyme, a mediator, a tetrazolium salt and an oxidizing agent selected from the group consisting of sodium chlorate, 2,5-dimethylhexane-2,5-dihydroperoxide, benzoyl peroxide, t-butyl peroxide, sodium iodate, N-ethylmaleimide, t-butylperoxyacetate, nickel acetylacetonate, stannic chloride, rhodium (III) trichloride hydrate, and t-butylperbenzoate is described. Inclusion of an oxidizing agent from the group specified above stabilizes the reagent, thereby preventing a high blank reaction because a high blank introduces error into the assay of an analyte. The reagent may be provided in solution form by adding water, or may be included in a film by adding a film-forming agent. The reagent may be utilized to perform the assay of an analyte from a fluid sample. The assay may be conducted in a solution medium by adding the fluid sample to the liquid reagent, and spectrophotometrically measuring absorbance of the color indicating the presence of formazan which was produced by reduction of the tetrazolium salt under assay conditions. Alternatively, the assay may be conducted in a film medium by adding the fluid sample to the reagent incorporated in the film, and spectrophotometrically measuring reflectance or transmittance of the color indicating formazan.

Stabilizing tetrazolium salts in a reagent

Control reagents an oxidant or a hydroxylamine compound together with other ingredients, the most important of which is a known amount of the analyte for which the reagent acts as a control.

Control reagent containing a hydroxylamine or an antioxidant

The invention relates to a non-serum based control reagent for glucose determination. Rather than using modified serum, the control reagent contains water, glucose, and the viscosity agent polystyrene sulphonate. The control reagent may also contain a buffer, preservatives, surfactants or surface active agents. A method of making the control reagent is also disclosed.

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Greenfield, IN, United States of America

David Tabb