10X Genomics, Inc.

University of California

David Sukovich

Augusto Manuel Tentori

Hanyoup Kim

Layla Katiraee

Yifeng Yin

Jerald Sapida

Lauren Gutgesell

Janine Hensel

Seayar Mohabbat

Katherine Pfeiffer

Andrew D Price

Tobias Daniel Wheeler

Marlon Stoeckius

Zahra Kamila Belhocine

Josephine Lee

Christina Galonska

Francesca Meschi

Jennifer Chew

Cedric Uytingco

Joseph Francis Shuga

Rapolas Spalinskas

Mesruh Turkekul

Anna-Maria Katsori

Aleksandra Jurek

Octavian Marian Bloju

Patrick Roelli

Ryo Hatori

Adam R Abate

John Christopher Anderson

Kamila Belhocine

Luz Montesclaros

Felice Alessio Bava

Markus De Raad

Shea Thompson Lance

Spontaneous Rose McKnight Russell

Shaun Lim

Cyrus Modavi

Alexander Hermes

Denise Cheung

Naishitha Anaparthy

Amanda Janesick

Spontaneous Rose Mcknight Russell

The present disclosure provides methods and systems for sample preparation and/or analysis. Samples may be cells, or may be derived from one or more cells. Sample preparation may comprise conducting one or more reactions on a target. Such reactions may be conducted in one or more partitions. One or more reactions may be performed in one or more successive operations.

Methods and systems for sample preparation and analysis

Provided herein are methods for capturing a connected probe and/or a capture handle sequence to a capture domain of a capture probe.

Methods, compositions, and systems for spatial analysis of analytes in a biological sample

Methods, compositions, and systems for capturing probes and/or barcodes

Provided herein are methods, compositions, and systems for improved in situ detection of analytes and spatial analysis using, e.g., a sequencing readout.

Methods for improved in situ detection of nucleic acids and spatial analysis

Provided herein are methods of enhancing spatial resolution of an analyte using sandwich maker system. The methods and systems used herein include a first substrate that includes a plurality of probes that include a capture domain and a spatial domain and a second substrate that includes a plurality of probes comprising a capture domain and a spatial domain.

Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis

Provided herein are methods, compositions, and kits for preparing biological samples for multiplex spatial gene expression and proteomic analysis, such as determining a location of a nucleic acid analyte and a protein analyte in a biological sample.

Multiplex capture of gene and protein expression from a biological sample

The methods and systems described herein provide an improved emulsion droplet based nucleic acid amplification method, which allows nucleic acids contained in biological systems to be detected, quantitated and/or sorted based on their sequence as detected with nucleic acid amplification techniques, e.g., polymerase chain reaction (PCR). The nucleic acids can be free floating or contained within living or nonliving structures, including particles, viruses, and cells. The nucleic acids can include, e.g., DNA or RNA.

Combined multiple-displacement amplification and PCR in an emulsion microdroplet

Traditional enzyme characterization methods are low-throughput, and therefore limit engineering efforts in synthetic biology and biotechnology. Here we propose a DNA-linked enzyme-coupled assay (DLEnCA) to monitor enzyme reactions in a high-throughput manner. Throughput is improved by removing the need for protein purification and by limiting the need for liquid chromatography mass spectrometry (LCMS) product detection by linking enzymatic function to DNA modification. DLEnCA is generalizable for many enzymatic reactions, and here we adapt it for glucosyltransferases, methyltransferases, and oxidoreductases. The assay utilizes cell free transcription/translation systems to produce enzymes of interest, while UDP-Glucose and T4-&#x3b2;-glucosyltransferase are used to modify DNA, which is detected post-reaction using qPCR or similar means of DNA analysis. For monitoring methyltransferases, consumption of SAM is observed by coupling to EcoRI methyltransferase. For monitoring oxidoreductases, consumption of NADH is observed by coupling to Taq orDNA ligase. OleD and two glucosyltransferases fromwere used to verify the assay's generality toward glucosyltransferases. Two methyltransferases from human andwere used to verify the assay's generality towards methyltransferases. We show DLEnCA's utility by mapping out the substrate specificity for these enzymes and observing the multiple steps of a biosynthetic pathway.

DNA-linked enzyme-coupled assays

Growing community of inventors

San Francisco, CA, United States of America

David Sukovich