University of Nebraska

J.a. Woollam Co.

David S Hage

Hai Xuan

William A Clarke

Abby Jackson

Chunling Wa

Craig M Herzinger

John A Woollam

Tino Hofmann

Mathias M Schubert

Stefan Schoeche

Erika Pfaunmiller

Ellipsometers and polarimeters or the like to investigate analyte containing fluids applied to a substrate-stage having a multiplicity of nano-structures that project non-normal to a surface thereof, including dynamics of interaction therewith, to the end of evaluating and presenting at least partial Jones or Mueller Matricies corresponding to a multiplicity of locations over an imaged area.

Birefringence imaging chromatography based on highly ordered 3D nanostructures

The present invention is directed to restricted access media (RAM), methods for preparing restricted access media, and kits for preparing restricted access media that contain protected ligand binding agents or protected enzymes. Certain RAM provided contain a plurality of protected regions of the support that contain ligand binding agents that are protected by blocking agents. Certain RAM provided contain a plurality of protected regions of the support that contain unbound ligand binding agents or enzymes that are retained in the protected regions by a capping agent. Methods of making the RAM of the invention and associated kits are also provided.

Restricted access media and methods for making restricted access media

A method and kit for immobilization of a glycoprotein. The method may include activating an affinity support. The affinity support may be activated by reacting the affinity support with a compound that is reactive with one or more functional groups included within the glycoprotein. The method may also include oxidizing the glycoprotein in which oxidation conditions are selected to yield an oxidized glycoprotein that is biologically active and contains a sufficient number of reactive aldehyde groups for coupling to a support. For example, the oxidized glycoprotein may include five reactive aldehyde groups. In addition, the method may include reacting the oxidized-glycoprotein with the activated affinity support to immobilize the glycoprotein.

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The invention is generally directed toward an analytical method to determine the concentration of an analyte or free analyte fraction in a sample. More particularly, the method encompasses applying a sample containing an analyte to an immunoaffinity column capable of selectively extracting the analyte in the millisecond time domain. The concentration of the analyte is then determined by chromatographic immunoassay.

Microcolumn chromatographic immunoassays

The invention is generally directed toward an analytical method to determine the concentration of the free analyte fraction in a sample. More particularly, the method encompasses applying a sample comprising a free and bound analyte fraction to an affinity column capable of selectively extracting the free fraction in the millisecond time domain. The signal generated by the free fraction is then quantified by standard analytical detection techniques. The concentration of the free fraction may then be determined by comparison of its signal with that of a calibration curve depicting the signal of known concentration of the same analyte.

Analysis of free analyte fractions by rapid affinity chromatography

The present invention generally relates to a microcolumn capable of separating an analyte from a sample in the millisecond time domain. The microcolumn is capable of such rapid separation by employing small column volumes that can tolerate medium to high flow rates. The invention also relates to a method of loading a microcolumn capable of separating an analyte from a sample in the millisecond time domain using plural injections of the packing material.

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Hickman, NE, United States of America

David S Hage