Michigan State University

Rohm and Haas Company

David Ross Dilley

Toni M Warner

Julian J Lee

Mikal E Saltveit, Jr

Zhenyong Wang

Dina K Kadyrzhanova

Edward Charles Kostansek

Richard M Basel

Robert Lynn Oakes

Eduardo José Bardella

Arden Nathan Reed

Jon Frederick Fobes

Richard Martin Basel

Eduardo Jose Bardella

Provided are methods of treating specific crop plants when those plants have reached specific developmental stages. Also provided is a method for improving the yield of a crop produced by a plurality of plants, which may or may not be any of the specific plants mentioned herein above, wherein said method comprises contacting said plants with at least one composition that comprises at least one cyclopropene.

Contacting crop plants with compositions

The present invention relates to a modified enzyme of a non-heme iron (II) dependent family of oxygenases and oxidases which renders the enzyme dependent on bicarbonate for activity. In a preferred embodiment, the modification is an arginine, lysine, or other amino acid that is two amino acid residues upstream of a histidine residue that is an iron ligand in the enzyme and is one of the histidine residues of the 2-histidine-1-aspartic acid trifacial motif. In particular, the modified enzymes are isopenicillin N synthetase, deacetoxycephalosporin C synthetase, and deacetoxycephalosporin C synthetase/deacetylcephalosporin C synthetase which are used to make antibiotics. The present invention further provides a method for making antibiotics using a modified enzyme such as isopenicillin N synthetase, deacetoxycephalosporin C synthetase, and deacetoxycephalosporin C synthetase/deacetylcephalosporin C synthetase wherein the modification renders the enzyme dependent on bicarbonate for activity. Finally, the present invention provides a method for making antibiotics by providing the modified enzyme either in either an organism or in a cell-free system.

Modified synthetases to produce penicillins and cephalosporins under the control of bicarbonate

A sensing apparatus and method for qualitatively and quantitatively analyzing small amounts of selected oxidizable gases in a carrier gas including the associated electronic circuitry is described. The apparatus preferably includes two semi-conductor type sensing elements in electrical parallel so that the difference in resistance of the two sensors can be detected, which can be of the N or P type and which decrease or increase in electrical resistance as a function of the concentration of an oxidizable gas. Also, a method for determining fruit maturity and ripeness by the detection of ethylene in the internal atmosphere of a fruit is described which preferably uses the apparatus with the semi-conductor type sensing elements, but can use other variable resistance type gas sensing means.

Gas sensing apparatus and method

Growing community of inventors

East Lansing, MI, United States of America

David Ross Dilley