Tufts College

Quanterix Corporation

Tufts University

Illumina Corporation

Optical Sensors, Inc.

Seventh Sense Biosystems, Inc.

Other

The Brigham and Women's Hospital, Inc.

Tuft College

David R Walt

David M Rissin

David C Duffy

Mark S Chee

David Fournier

Todd Alan Dickinson

Cheuk Wai Kan

Karri L Michael

Steven Mark Barnard

Stanley M Klainer

Karri Lynn Michael-Ballard

Amos J Gottlieb

Laura Taylor

Brian G Healey

Howard Bernstein

Donald E Chickering, Iii

Douglas Adam Levinson

Ramin Haghgooie

Shawn Davis

Jeffrey D Randall

Sandra Bencic-Nagale

Evan Ferrell

Robert Samuel Langer

Irving L Weissman

Frank J Steemers

David James Monk

Michael S Fleming

Linan Song

Daniel B Raemer

Lei Chang

Karri Lynn Ballard

Sabine Szunerits

Mary Beth Tabacco

Michael R Webb

John S Kauer

Christopher Lafratta

Manuel Alfredo Palacios

Jenny Tam

Michael Lacy

Tarun K Mandal

Mahesh Uttamlal

Christianne Munkholm

Steven M Bernard

Karen S Bronk

Paul Pantano

Hans-Heiner Gorris

Suneet Chadha

Alexei R Faustov

Shazia Baig

Stephanie Schubert

Timothy M Blicharz

Caroline L Schauer

Israel Biran

The present invention relates to systems and methods for detecting analyte molecules or particles in a fluid sample and in some cases, determining a measure of the concentration of the molecules or particles in the fluid sample. Methods of the present invention may comprise immobilizing a plurality of analyte molecules or particles with respect to a plurality of capture objects. At least a portion of the plurality of capture objects may be spatially separated into a plurality of locations. A measure of the concentration of analyte molecules in a fluid sample may be determined, at least in part, on the number of reaction vessels comprising an analyte molecule immobilized with respect to a capture object. In some cases, the assay may additionally comprise steps including binding ligands, precursor labeling agents, and/or enzymatic components.

Ultra-sensitive detection of molecules or particles using beads or other capture objects

The invention provides methods and compositions for distinguishing breast cancer.

Compositions and methods for diagnosing breast cancer

Described herein are systems and methods for extending the dynamic range of assay methods and systems used for determining the concentration of analyte molecules or particles in a fluid sample. In some embodiments, a method comprises spatially segregating a plurality of analyte molecules in a fluid sample into a plurality of locations. At least a portion of the locations may be addressed to determine the percentage of said locations containing at least one analyte molecule. Based at least in part on the percentage, a measure of the concentration of analyte molecules in the fluid sample may be determined using an analog, intensity-based detection/analysis method/system and/or a digital detection/analysis method/system. In some cases, the assay may comprise the use of a plurality of capture objects.

Methods and systems for extending dynamic range in assays for the detection of molecules or particles

Arrays of single molecules and methods of producing an array of single molecules are described. Arrays with defined volumes between 10 attoliters and 50 picoliters enable single molecule detection and quantitation.

Methods and arrays for target analyte detection and determination of target analyte concentration in solution

Methods and arrays for target analyte detection and determination of target  analyte concentration in solution

A microsphere-based analytic chemistry system and method for making the same is disclosed in which microspheres or particles carrying bioactive agents may be combined randomly or in ordered fashion and dispersed on a substrate to form an array while maintaining the ability to identify the location of bioactive agents and particles within the array using an optically interrogatable, optical signature encoding scheme. A wide variety of modified substrates may be employed which provide either discrete or non-discrete sites for accommodating the microspheres in either random or patterned distributions. The substrates may be constructed from a variety of materials to form either two-dimensional or three-dimensional configurations. In a preferred embodiment, a modified fiber optic bundle or array is employed as a substrate to produce a high density array. The disclosed system and method have utility for detecting target analytes and screening large libraries of bioactive agents.

Target analyte sensors utilizing microspheres

Methods for detecting target analytes and enzymatic reactions

Disclosed is a method for measuring the concentration of an analyte or analytes in a solution. Although the methods can be conducted using a number of different assay formats, in one embodiment, the assays are conducted in reaction vessels defined, at least in part, by the distal ends of fiber optic strands.

Methods for determining the concentration of an analyte in solution

The present invention generally relates to devices and techniques associated with diagnostics, therapies, and other applications, including skin-associated applications, for example, devices for delivering and/or withdrawing fluid from subjects, e.g., through the skin. In some embodiments, the device includes a system for accessing an extractable medium from and/or through the skin of the subject at an access site, and a pressure regulator supported by a support structure, able to create a pressure differential across the skin at at least a portion of the access site. The device may also include, in some cases, a sensor supported by the support structure for determining at least one condition of the extractable medium from the subject, and optionally a signal generator supported by the support structure for generating a signal relating to the condition of the medium determined by the sensor.

Devices and techniques associated with diagnostics, therapies, and other applications, including skin-associated applications

Described herein are systems and methods for the detection of and/or determination of a measure of the concentration of analyte molecules or particles in a fluid sample. In some cases, the systems and methods employ techniques to reduce or limit the negative effects associated with non-specific binding events. Certain methods of the present invention involve associating the analyte molecules at least a first type of binding ligand and at least a second type of binding ligand, and spatially segregating the analyte molecules into a plurality of locations on a surface. The presence of an analyte molecule at or in a location may be determined by determining the presence of both the first type of binding ligand and the second type of binding ligand.

Ultra-sensitive detection of molecules using dual detection methods

The present disclosure, among other things, methods and systems for digital quantification of single molecule analytes.

Digital quantification of single molecules

Growing community of inventors

Boston, MA, United States of America

David R Walt