United States of America, As Represented by the Secretary of Agriculture

The United States of America As Represented by the Secretary of

David R Rockhold

William R Belknap

Kent F McCue

Paul V Allen

Mary M Maccree

Howard V Davies

Nathaniel T Taylor

Louise V T Shepherd

Sophie S Chang

Mendel Friedman

A Douglas King, Jr

Charles P Moehs

Teruko Osumi

Kenneth L Deahl

Christina Royer

Tamas Torok

Andrew Stapleton

The present invention relates to isolatedBul409 promoter sequences and uses thereof. An exemplary embodiment provides an isolated plant Bul409 promoter comprising a nucleic acid sequence that is at least about 90% identical to nucleotides 1-771 of SEQ ID NO:1, wherein the promoter sequence is capable of controlling transcription in a plant. Other exemplary embodiments provide a method for making a transgenic plant, wherein the method comprises transforming a plant, plant part, or plant cell with an expression vector comprising isolated plant Bul409 promoter operably linked to a heterologous nucleic acid sequence, wherein the isolated plant Bul409 promoter is capable of controlling transcription of the heterologous nucleic acid in a plant, and a transgenic plant made by the method and decendants thereof.

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The present invention relates to isolatedBul427 promoter sequences and uses thereof. An exemplary embodiment provides an isolated plant Bul427 promoter comprising a nucleic acid sequence that is at least about 90% identical to nucleotides 1-1154 of SEQ ID NO:1, wherein the promoter sequence is capable of controlling transcription in a plant. Other exemplary embodiments provide a method for making a transgenic plant, wherein the method comprises transforming a plant, plant part, or plant cell with an expression vector comprising isolated plant Bul427 promoter operably linked to a heterologous nucleic acid sequence, wherein the isolated plant Bul427 promoter is capable of controlling transcription of the heterologous nucleic acid in a plant, and a transgenic plant made by the method and decendants thereof.

Nucleic acid sequences from potato that encode the enzyme &#x3b2;-solanine/&#x3b2;-chaconine rhamnosyltransferase (SGT3) are disclosed. Recombinant DNA molecules containing the sequences, and use thereof, in particular, use of the sequences and antisense constructs to inhibit the production of SGT3 and thereby reduce levels of the predominant steroidal glycoalkaloids &#x3b1;-chaconine and &#x3b1;-solanine in Solanaceous plants such as potato are described.

Nucleic acid sequences from potato that encode the enzyme UDP-glucose:solanidine glucosyltransferase (SGT2) are disclosed. Recombinant DNA molecules containing the sequences, and use thereof, in particular, use of the sequences and antisense constructs to inhibit the production of SGT2 and thereby reduce the level of the more human-toxic of the two predominant steroidal glycoalkaloids &#x3b1;-chaconine and/or increase the level of the more insect-toxic &#x3b1;-solanine in Solanaceous plants such as potato are described.

Agene has been cloned from a late blight-resistance locus and its sequence provided, together with the sequence of the encoded protein. DNA encoding the resistance protein has been introduced into potato plants and confers resistance to, the causal agent of late blight. The resistance protein is in the class of Nucleotide Binding Site-Leucine-Rich Repeat Proteins (NBS-LRRP), and the gene inis flanked by related NBS-LRRP gene sequences.

DNA sequences from potato which encode the enzyme solanidine UDP-glucose glucosyltransferase (SGT) are disclosed. Recombinant DNA molecules containing the sequences, and use thereof, in particular, use of an antisense DNA construct to inhibit the production of SGT and thereby reduce glycoalkaloid levels in solanaceous plants, e.g., potato, are described.

DNA sequences from potato encoding solanidine UDP-glucose

A species-specific DNA-DNA hybridization probe prepared using an isolated whole chromosome of a lower eukaryote as template. The probe is useful for the detection of conspecificity in lower eukaryotes selected from the group consisting of yeast and molds.

Growing community of inventors

El Cerrito, CA, United States of America

David R Rockhold