J. T. Baker Chemical Company

The Procter Gamble Company

J. T. Baker, Inc.

David R Nau

Hugh E Ramsden

Laura J Crane

Bernard William Kluesener

Adam W Mazur

A method of isolating and purifying galactose oxidase from a fungus media is disclosed. The supernatant liquid from the fermentation broth is subjected to ultrafiltration and then separated on a carboxy-sulfon cation exchange column using high performance liquid chromatography. Copper ions must be present to insure enzyme activity.

Process for isolating galactose oxidase

Sulfonic derivatives of N-acylated covalently bound, non-crosslinked polyethyleneimine bonded phase silicas of the formula: ##STR1## wherein ##STR2## is the backbone of a silica gel or glass, n is an integer such that the polyethyleneiminopropyl silane group has an average molecular weight of from about 400 to about 1800; q is an integer of zero or one; R is selected from the group consisting of hydrogen, --CH.sub.2).sub.x H or --CH.sub.2).sub.m COOH where X is an integer of 1 or 2 and m is an integer of zero or 1; when R is hydrogen or --CH.sub.2).sub.x H then R.sup.1 is --CH.sub.2).sub.p COOH where p is an integer of zero or 1, and when R is --CH.sub.2).sub.m COOH then R.sup.1 is selected from the group consisting of hydrogen or --CH.sub.2).sub.y H where y is an integer of zero or 1, are especially useful as solid phases for the purification and separation of proteins having isoelectric points of below about 5.

N-acylated derivatives of polyethyleneimine bonded phase silica products

Sulfonic derivatives of N-acylated covalently bound, non-crosslinked polyethyleneimine bonded phase silicas of the formula: ##STR1## wherein is the backbone of a silica gel or glass, n is an integer such that the polyethyleneiminopropyl silane group has an average molecular weight of from about 400 to about 1800; q is an integer of zero or one; R is selected from the group consisting of hydrogen, --CH.sub.2).sub.x H or --CH.sub.2).sub.m COOH where x is an integer of 1 or 2 and m is an integer of zero or 1; when R is hydrogen or --CH.sub.2).sub.x H then R.sup.1 is --CH.sub.2).sub.p COOH where p is an integer of zero or 1, and when R is --CH.sub.2).sub.m COOH then R.sup.1 is selected from the group consisting of hydrogen or --CH.sub.2).sub.y H where y is a integer of zero or 1, are especially useful as solid phases for the purification and separation of proteins having isoelectric points of below about 5.

Use of sulfonic derivatives of acylated polyethyleneimine bonded phase

Sulfonic derivatives of N-acylated covalently bound, non-crosslinked polyethyleneimine bonded phase silicas of the formula: ##STR1## is the backbone of a silica gel or controlled pore glass, n is an integer such that the polyethyleneiminopropyl silane group has an average molecular weight of from about 400 to about 1800; q is an integer of zero or one; R is selected from the group consisting of hydrogen, --(CH.sub.2).sub.x H or --(CH.sub.2).sub.m COOH where x is an integer of 1 or 2 and m is an integer of zero or 1; when R is hydrogen or --(CH.sub.2).sub.x H then R.sup.1 is --(CH.sub.2).sub.p COOH where p is an integer of zero or 1, and when R is --(CH.sub.2).sub.m COOH then R.sup.1 is selected from the group consisting of hydrogen or --(CH.sub.2).sub.y H where y is an integer of zero or 1, are especially useful as solid phases for the purification and separation of proteins having isoelectric points of below about 5.

Sulfonic derivatives of acylated polyethyleneimine bonded phase silica

A method of separating and recovering immunoglobulin G from biological fluids by liquid chromatographic or solid phase extraction processes utilizing, as the solid phase, a carboxylated form of silica gel or controlled pore glass bearing non-cross-linked covalently bound polyethylenimine functions.

Growing community of inventors

Lebanon, NJ, United States of America

David R Nau