Monsanto Technology LLC

David R Duncan

Brenda A Lowe

Jyoti R Rout

Michael T Mann

Anisha Akula

William L Petersen

David D Songstad

Wanggen Zhang

Joel B Wilks

Cristina Ubach

Brian J Martinell

Xudong Ye

Guangning Ye

Anatoly Rivlin

Charles L Armstrong

Luis Jurado

Vladimir Sidorov

Yurong Chen

Maria Cristina Ubach

Ashok Shrawat

David Kelm

Sarah Heifner

Rebecca Rode

Lorena Moeller

A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.

Methods for producing transgenic plants

The present invention provides methods for the transformation of viable explants from wheat seeds to permit production of transgenic wheat plants. The present invention also relates to methods for producing such explants and related embodiments.

Methods for transforming wheat explants and compositions therefor

Production of transformed plants is achieved by placing-inoculated explants directly onto a medium containing selective agents for suppressing growth ofand killing non-transformed explant cells.

Methods and compositions are provided for spatial imaging and quantifying glyphosate in plant tissue. Glyphosate oxidoreductase is coupled to a cycling flavin mononucleotide-oxidoreductase-luciferase system. The resulting bioluminescence is proportional to the amount of glyphosate, allowing glyphosate to be observed within plant tissue and quantified.

Imaging glyphosate in plant tissue

Use of a low-oxygen environment before the transformation process can enhance transformation of plants. Low oxygen during regeneration can also increase the culturability of recalcitrant genotypes.

Use of a low-oxygen environment in plant transformation

Methods for the regeneration of cotton plants are disclosed. The use of novel compositions of media, media additives and new growth conditions during stages of development results in increased frequencies of embryogenesis, embryo maturation and embryo germination. The improved process results in higher production frequencies of transformed cotton plants.

Methods for inducing cotton embryogenic callus

Use of nitric oxide modulators during the transformation process can enhance-mediated transformation of plants.

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The present invention relates to a novel system for generating transformed dihaploid plants from haploid cells and tissues without the use of chromosome doubling agents.

Method for agrobacterium transformation for dihaploid corn plants

Growing community of inventors

St. Charles, MO, United States of America

David R Duncan