The Upjohn Co.

Pharmacia & Upjohn Company

David P Brunner

Richard J Kirschner

Robert L Garlick

John E Mott

Gary C Harbour

Frances M Eckenrode

James F Pinner

Disclosed are expression vectors useful as vectors in recombinant methods to facilitate expression of exogenous genes in E. coli. Specifically, the disclosed expression vector has the following elements in operable linkage: the PL6m promoter, the TAT32 ribosome binding site and a gene encoding a heterologous polypeptide, Also disclosed are E. coli host cells transformed with this expression vector.

Expression vector containing PL6M promoter and TAT32 ribosome binding

The present invention provides an inexpensive fermentation medium for growing microorganisms. Also provided are fermentation media and methods for producing norleucine by growing E. coli thereon, fermentation media and methods for incorporating norleucine into polypeptides expressed by microorganisms grown thereon, and fermentation media and methods for preventing the incorporation of norleucine into polypeptides expressed by microorganisms grown thereon. Also provided are bovine somatotropin analogs.

Fermentation media and methods for controlling norleucine in polypeptides

Fermentation method for producing norleucine

The present invention is based upon the discovery that proteins made from genes that include the CD4 sequence in its cDNA can make additional polypeptides as a result of internal translation initiation. This invention is thus directed to DNA sequences which eliminate internal initiation expression in sCD4.

Eliminating internal initiation of soluble CD4 gene

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Portage, MI, United States of America

David P Brunner