The United States of America as Represented by the Secretary of

Washington State University

United States of America, as Represented by the Secretary of Agriculture

Other

David M Weller

Linda S Thomashow

R James Cook

Dmitri V Mavrodi

Shannon M Delaney

Jos M Raaijmakers

Mahalaxmi Bangera

Zhengyu Huang

Henry T Wilkinson

Dal-Soo Kim

Mingming Yang

Jibin Zhang

J Ole Becker

In exemplary embodiments, the invention provides2-79 strains having pyrrolnitrin biosynthetic genes for biocontrol of soilborne diseases.

[object Object]

Th invention is directed to nucleic acid sequences which encode polypeptides having PhzO activity, namely, the ability to convert phenazine-1-carboxylic acid to a 2-hydroxylated phenazine, and isolated polypeptides having this activity. The invention is also directed to recombinant nucleic acid molecules, vectors, and host cells including the nucleic acid sequences as well as methods for producing and using the polypeptides, including expression in bacterial or plant cells to inhibit fungal pathogens.

Sequences encoding PhzO and methods

The invention is directed to nucleic acid sequences which encode polypeptides having PhzO activity, namely, the ability to convert phenazine-1-carboxylic acid to a 2-hydroxylated phenazine, and isolated polypeptides having this activity. The invention is also directed to recombinant nucleic acid molecules, vectors, and host cells including the nucleic acid sequences as well as methods for producing and using the polypeptides, including expression in bacterial or plant cells to inhibit fungal pathogens.

Fluorescent Pseudomonas spp. are described which are effective for the control of diseases caused by the soil-borne fungus, ( ), such as take-all, in small grain crops or turf grass. The subject biocontrol strains have a unique genotype as shown by a characteristic banding pattern, and exhibit root-colonizing ability which is characterized by both higher population density on roots and extended colonizing activity compared to known Gg-suppressive strains. A further property is the ability of a strain to duplicate the level of biocontrol obtained naturally in a take-all decline soil. Methods for isolation and identification of the strains and their use to control diseases caused by Gg are provided. In particular, strains of NRRL B-21806 and NRRL B-21807.

Biocontrol agents for take-all

Transgenic fluorescent Pseudomonas spp. are described which have a biosynthetic locus which encodes for the production of the antibiotic phenazine-1-carboxylic acid stably introduced into the genome, have a locus which encodes for the production of the antibiotic 2,4-diacetylphloroglucinol, and are effective for control of diseases caused by the soil-borne fungus, Rhizoctonia. Strains are also described which control diseases caused by,or Pythium, in addition to Rhizoctonia, or have the ability to control all three diseases.

Transgenic strains of Pseudomonas for biocontrol of plant root diseases

DNA sequences which function specifically in the synthesis of 2,4-diacetylphloroglucinol (Phl) are described. The sequences include phl genes which encode phl gene proteins and coding and regulatory sequences for production of Phl as well as sequences containing phl genes, which sequences have the capability of conferring or enhancing Phl biosynthetic capability in bacterial strains. The transformed strains are useful as biocontrol agents against fungal pathogens.

Sequences for production of 2,4-diacetylphloroglucinol and methods

Methods and products useful to simultaneously control the root diseases caused by Gaeumannomyces graminis, Rhizoctonia, and Pythium in crops susceptible to these root diseases are described. These include a method to identify seed-treatment products for potential to simultaneously control the three kinds of root diseases; Bacillus strains having activity against the three kinds of root diseases and methods of obtaining the same, and compositions comprising a fungicide and a Bacillus strain having activity against the three kinds of root diseases, which compositions are useful for field control of the combination of the three kinds of root diseases.

Methods and compositions for the simultaneous control of the root

Sequences for production of 2,4-Diacetylphloroglucinol and methods

A method for screening bacteria to select strains which will suppress Pythium spp. in small grain crops under field conditions and a method for applying field-suppressive bacteria to suppress Pythium spp. in a commercial setting are described. Four Pseudomonas strains which passed the screen test are disclosed.

Method for screening bacteria and application thereof for field control

A method for screening bacteria to select strains which will suppress diseases caused by the fungus Gaeumannomyces graminis (Gg) under field conditions and a method for applying field-suppressive bacteria to suppress Gg in a commercial setting. Four fluorescens Pseudomonas strains are disclosed which are effective in suppressing take-all in wheat.

Growing community of inventors

Pullman, WA, United States of America

David M Weller