Monsanto Company

Monsanto Technology LLC

Monsato Company

Holganix, LLC

David M Stark

Ganesh M Kishore

Gerard Francis Barry

Timothy A Mitsky

Stephen Rogers Padgette

Steven C Slater

Kenneth Gruys

Kenneth James Gruys

Steven Charles Slater

Salim M Hakimi

Janice W Edwards

James Conrad Zalewski

Bradley M Krohn

Thomas E Clemente

Robert Neidermyer

Methods for controlling pathogenic plant nematodes using organic soil amendments applied to soil and/or plants, methods of improving the performance and/or improving the health of a ruminant animal and methods of decreasing nematodes in a ruminant animal are described.

Methods for controlling pathogenic plant nematodes and for improving the health and/or performance of ruminant animals

Nucleic acid sequences, vectors, recombinant host cells, transgenic plants, and methods for their preparation are disclosed. The nucleic acid sequences encode threonine deaminase proteins that catalyze the conversion of threonine to &#x3b1;-ketobutyrate. One or both of the amino acids at positions 447 and 481 of the encoded proteins can be selected from groups of particular amino acids. For example, the amino acid at position 447 can be alanine, isoleucine, valine, phenylalanine, tryptophan, or methionine. The amino acid at position 481 can be alanine, isoleucine, proline, phenylalanine, tryptophan, or methionine.

Modified threonine deaminase

The present invention relates to a method for enhancing a plant's resistance to diseases and/or bruising by transforming the plant with sense and antisense polyphenol oxidase encoding sequences from potato. The invention also relates to transgenic plants and progeny thereof with reduced polyphenol oxidase activity.

Method of imparting disease resistance to plants by reducing polyphenol oxidase activities

Nucleic acid encoding a modified threonine deaminase and methods of use

Promoters for enhanced expression of ADPglucose pyrophosphorylase in potato tubers and fruits such as tomato; methods of using them; DNA molecules, plant cells and plants containing them. A method of decreasing the oil content of seeds by expression of ADPglucose pyrophosphorylase.

Enhanced starch biosynthesis in seeds

Genes and methods for optimizing levels of substrates employed in the biosynthesis of copolymers of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) in plants and bacteria via manipulation of normal metabolic pathways using recombinant DNA techniques are provided. This is achieved through the use of a variety of wild-type and/or deregulated enzymes involved in the biosynthesis of aspartate family amino acids, and wild-type or deregulated forms of enzymes, such as threonine deaminase, involved in the conversion of threonine to P(3HB-co-3HV) copolymer endproduct. By these methods, enhanced levels of threonine, .alpha.-ketobutyrate, propionate, propionyl-CoA, .beta.-ketovaleryl-CoA, and .beta.-hydroxyvaleryl-CoA are produced. Also provided are methods for the biological production of P(3HB-co-3HV) copolymers in plants and bacteria utilizing propionyl-CoA produced through a variety of engineered metabolic pathways. Introduction into plants and bacteria of an appropriate .beta.-ketothiolase, .beta.-ketoacyl-CoA reductase, and PHA synthase, alone or in combination with various enzymes involved in asparate family amino acid biosynthesis and the conversion of threonine to PHA copolymer precursors, will permit these organisms to produce P(3HB-co-3HV) copolymers.

Methods of optimizing substrate pools and biosynthesis of

Genes and methods for optimizing levels of substrates employed in the biosynthesis of copolymers of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) in plants and bacteria via manipulation of normal metabolic pathways using recombinant DNA techniques are provided. This is achieved through the use of a variety of wild-type and/or deregulated enzymes involved in the biosynthesis of aspartate family amino acids, and wild-type or deregulated forms of enzymes, such as threonine deaminase, involved in the conversion of threonine to P(3HB-co-3HV) copolymer end product. By these methods, enhanced levels of threonine, .alpha.-ketobutyrate, propionate, propionyl-CoA, .beta.-ketovaleryl-CoA, and .beta.-hydroxyvaleryl-CoA are produced. Also provided are methods for the biological production of P(3HB-co-3HV) copolymers in plants and bacteria utilizing propionyl-CoA produced through a variety of engineered metabolic pathways. Introduction into plants and bacteria of an appropriate .beta.-ketothiolase, .beta.-ketoacyl-CoA reductase, and PHA synthase, alone or in combination with various enzymes involved in asparate family amino acid biosynthesis and the conversion of threonine to PHA copolymer precursors, will permit these organisms to produce P(3HB-co-3HV) copolymers.

Isolated DNA fragments encoding a Rhodospirillum rubrum (ATCC 25903) polyhydroxyalkanoate (PHA) synthase, or biologically functional equivalents thereof, are provided. Also provided is the deduced amino acid sequence of the R. rubrum PHA synthase. These molecules are useful in the production of PHAs in bacteria and plants.

Rhodospirillum rubrum poly-.beta.-hydroxyalkanoate synthase

Method of improving the quality of stored potatoes

Enhanced starch biosynthesis in tomatoes

Promoters isolated from potato which cause expression of a gene of choice in tomato; tomato plant cells and plants containing them.

Growing community of inventors

Chesterfield, MO, United States of America

David M Stark