University of California

Aviva Biosciences Corporation

Tsinghua University

Arrayomics, Inc.

Captialbio Corporation

David M Rothwarf

Xiaobo Wang

Michael Karin

Lei Wu

Ebrahim Zandi

Junquan Xu

Joseph A DiDonato

Mingxian Huang

Makio Hayakawa

Jing Cheng

Guoliang Tao

Litian Liu

Weiping Yang

Depu Chen

Wei Shao

Baoquan Sun

Guo Liang Tao

Bao Vinh Nguyen

Jia Xu

Christopher D Herold

Antonio Guia

Mingxiang Huang

Microdevices containing a predetermined preferential axis of magnetization are disposed in an array having discreet regions. Under influence of a magnetic field, the microdevices can have at least twelve discrete orientations, and can advantageously be flipped upside down in place. Microdevices can be coded in a manner that supports a coding space of at least 10, 10, 10or even 10or more choices, and can include one or more chemically reactive sites. The regions can be defined by long and short bars, in which microdevices span gaps between the longer bars, and the shorter bars measure less than 60% of such gaps. Preferred embodiments are also provided to produce microfabricated microdevices for magnetic assembly-based arraying.

Microdevice arrays formed by magnetic assembly

This invention relates generally to the field of moiety or molecule analysis, isolation, detection and manipulation and library synthesis. In particular, the invention provides a microdevice, which microdevice comprises: a) a substrate; and b) a photorecognizable coding pattern on the substrate. Preferably, the microdevice does not comprise an anodized metal surface layer. Methods and kits for isolating, detecting and manipulating moieties, and synthesizing libraries using the microdevices are also provided. The invention further provides two-dimensional optical encoders and uses thereof.

Microdevice containing photorecognizable coding patterns and methods of using and producing the same

The present invention recognizes that the determination of an ion transport function or property using direct detection methods, such as patch-clamps, whole cell recording or single channel recording, are preferable to methods that utilize indirect detection methods, such as FRET based detection system. The present invention provides biochips and methods of use that allow for the direct analysis of ion transport functions or properties using microfabricated structures that can allow for automated detection of one or more ion transport functions or properties. These biochips and methods of use thereof are particularly appropriate for automating the detection of ion transport functions or properties, particularly for screening purposes.

Biochips including ion transport detecting structures and methods of use

This invention relates generally to the field of moiety or molecule isolation, detection and manipulation and library synthesis. In particular, the invention provides a microdevice, which microdevice comprises: a) a magnetizable substance; and b) a photorecognizable coding pattern, wherein said microdevice has a preferential axis of magnetization. Systems and methods for isolating, detecting and manipulating moieties and synthesizing libraries using the microdevices are also provided.

Microdevices having a preferential axis of magnetization and uses thereof

The present invention provides an isolated nucleic acid molecules encoding I&#x3ba;B kinase (IKK) catalytic subunit polypeptides, which are associated with an IKK serine protein kinase that phosphorylates a protein (I&#x3ba;B) that inhibits the activity of the NF-&#x3ba;B transcription factor, vectors comprising such nucleic acid molecules and host cells containing such vectors. In addition, the invention provides nucleotide sequences that can bind to a nucleic acid molecule of the invention, such nucleotide sequences being useful as probes or as antisense molecules. The invention also provides isolated IKK catalytic subunits, which can phosphorylate an I&#x3ba;B protein, and peptide portions of such IKK subunit. In addition, the invention provides anti-IKK antibodies, which specifically bind to an IKK complex or an IKK catalytic subunit, and IKK-binding fragments of such antibodies. The invention further provides methods of substantially purifying an IKK complex, methods of identifying an agent that can alter the association of an IKK complex or an IKK catalytic subunit with a second protein, and methods of identifying proteins that can interact with an IKK complex or an IKK catalytic subunit.

I&#x3ba;B Kinase-&#x3b2; (IKK&#x3b2;) binding antibodies and methods of using same

The present invention provides a novel essential regulatory subunit of the I&#x3ba;B kinase (IKK) complex, IKK-&#x3b3;. The isolated IKK-&#x3b3; subunit of the invention has substantially the same amino acid sequence as SEQ ID NO: 2 shown in FIG.

Gamma subunit of cytokine responsive I&#x3ba;B-alpha kinase complex and methods of using same

The present invention provides an isolated nucleic acid molecules encoding I&kgr;B kinase (IKK) catalytic subunit polypeptides, which are associated with an IKK serine protein kinase that phosphorylates a protein (I&kgr;B) that inhibits the activity of the NF-&kgr;B transcription factor, vectors comprising such nucleic acid molecules and host cells containing such vectors. In addition, the invention provides nucleotide sequences that can bind to a nucleic acid molecule of the invention, such nucleotide sequences being useful as probes or as antisense molecules. The invention also provides isolated IKK catalytic subunits, which can phosphorylate an I&kgr;B protein, and peptide portions of such IKK subunit. In addition, the invention provides anti-IKK antibodies, which specifically bind to an IKK complex or an IKK catalytic subunit, and IKK-binding fragments of such antibodies. The invention further provides methods of substantially purifying an IKK complex, methods of identifying an agent that can alter the association of an IKK complex or an IKK catalytic subunit with a second protein, and methods of identifying proteins that can interact with an IKK complex or an IKK catalytic subunit.

I&kgr;B kinase, subunits thereof, and methods of using same

The present invention provides a substantially purified nucleic acid molecule encoding a serine protein kinase (I&kgr;B kinase) that phosphorylates a protein (I&kgr;B) that inhibits the activity of the NF-&kgr;B transcription factor, vectors comprising such a nucleic acid molecule and host cells containing such vectors. In addition, the invention provides nucleotide sequences that can bind to a nucleic acid molecule of the invention, such nucleotide sequences being useful as probes or as antisense molecules. The invention also provides a substantially purified I&kgr;B kinase, which is a polypeptide that can phosphorylate an I&kgr;B protein, and peptide portions of the I&kgr;B kinase. In addition, the invention provides anti-I&kgr;B kinase antibodies, which specifically bind to an I&kgr;B kinase, and I&kgr;B kinase-binding fragments of such antibodies. The invention further provides methods of substantially purifying an I&kgr;B kinase, methods of identifying an agent that can alter the association of an I&kgr;B kinase with a second protein, and methods of identifying proteins that can interact with an I&kgr;B kinase.

IKB kinase and methods of using same

The present invention provides an isolated nucleic acid molecules encoding I,B kinase (IKK) catalytic subunit polypeptides, which are associated with an IKK serine protein kinase that phosphorylates a protein (I,B) that inhibits the activity of the NF-,B transcription factor, vectors comprising such nucleic acid molecules and host cells containing such vectors. In addition, the invention provides nucleotide sequences that can bind to a nucleic acid molecule of the invention, such nucleotide sequences being useful as probes or as antisense molecules. The invention also provides isolated IKK catalytic subunits, which can phosphorylate an I,B protein, and peptide portions of such IKK subunit. In addition, the invention provides anti-IKK antibodies, which specifically bind to an IKK complex or an IKK catalytic subunit, and IKK-binding fragments of such antibodies. The invention further provides methods of substantially purifying an IKK complex, methods of identifying an agent that can alter the association of an IKK complex or an IKK catalytic subunit with a second protein, and methods of identifying proteins that can interact with an IKK complex or an IKK catalytic subunit.

IkB kinase, subunits thereof, and methods of using same

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La Jolla, CA, United States of America

David M Rothwarf