The United States of America as Represented by the Secretary of

North Carolina State University

David M Pharr

Roger L Thompson

Henry P Fleming

Johan M Stoop

A mannitol oxidoreductase is isolated from vascular plants which is NAD.sup.+ dependent and which converts mannitol to mannose. A preferred source of the mannitol oxidoreductase is Apium graveolens, particularly Apium graveolens L. var. rapaceum (Mill.) Gaud. (celeriac) and Apium graveolens L. var. dulce (Mill.) Pers. (celery). DNA encoding of the mannitol oxidoreductase may be used in the production of the enzyme.

Mannitol oxidoreductase isolated from vascular plants

Fresh whole produce is placed in a container and continuously purged for about 30 to 120 minutes with oxygen or carbon dioxide gas. The container is then filled with a brine solution and the produce allowed to stand for a period of about 2-24 hours, thus resulting in superior pickled produce.

Growing community of inventors

Raleigh, NC, United States of America

David M Pharr