E.i. Dupont De Nemours and Company

Dade International Inc.

David M Obzansky

Susan Y Tseng

William Jeffrey Allard

Donald M Simons

Hemant Chunilal Vaidya

Hermant C Vaidya

An assay for photometrically detecting and/or quantitating the presence of an analyte in a sample in which the signal generated by a label associated with the analyte is photometrically detected in the presence of a suspended solid support.

Assay with signal detection in the presence of a suspended solid support

An immunoassay for CK isoenzyme or CK isoform is provided based on capture of the CK isoenzyme or CK isoform by a specific antibody immobilized through a cleavable linker containing a disulfide bond onto a solid phase and release of the resulting antibody-CK isoenzyme or antibody-CK isoform complex by the addition of a reducing agent to cleave the disulfide bond and simultaneously activate the CK isoenzyme or CK isoform.

Determination of CK isoenzymes and CK isoforms

Immunoassays and DNA probe assays utilizing a non-immune, reversible binding displacement system are provided. In the assay, a releasable ligand, a binding partner for the releasable ligand, an analyte of interest, an analytically detectable (reporter) group, and at least one binding partner for the analyte, are first attached to an insoluble phase so as to form reporter-labeled complex bound to an insoluble phase, followed by the addition of a displacer ligand which displaces the releasable ligand along with some portion of the reporter-labeled complex, so that the released reporter is analytically detectable in a free liquid medium and can be related to the concentration of analyte in the sample.

Method for specific binding assays using a releasable ligand

In situ process for production of conjugates using a deblocking agent which allows conjugation to occur and two compositions wherein one composition is functionalized with a group capable of reacting efficiently with free thiols, and a second composition containing blocked thiols.

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Elkton, MD, United States of America

David M Obzansky