Abbott Laboratories Corporation

David M Finley

Martin Wong

Stephen D Stroupe

John M Brackett

Shelley R Holets-McCormack

Elaine Marie Brate

John M Ramp

Thomas G Spring

Mark R Shaffar

Philip P Wang

Gary L Boltinghouse, Jr

David P Pacenti

Phillip P Wang

Michelle L Shields

Ryan E Piktel

Shelley R Holets-Mccormack

Michelle Shields

The present disclosure provides among other things immunoassays exhibiting reduced cross-reactivity with analyte metabolites. Additionally, the present disclosure provides diagnostic immunoassays to determine the concentration or level in a test sample of a hydrophobic drug that metabolizes in vivo or in vitro to form cross-reacting metabolites wherein cross-reactivity with such metabolites of the drug analyte is reduced. In particular, the disclosure provides such immunoassays where the hydrophobic drug is an immunosuppressant drug such as cyclosporine A.

Immunoassays exhibiting reduced cross-reactivity with hydrophobic drug analyte metabolites

The present invention provides a method of improving the sensitivity and accuracy of a lead assay. The method enhances the recovery of lead during isolation of the lead from interfering compounds by maintaining the lead in a sample solution and making the recovered lead available for detection by the assay. An enhancing reagent complexes with the lead isolated in the sample solution. The enhancer includes a chelator having a lead equilibrium binding constant in the range of about 4 log K to about 13 log K. A kit for performing such a lead assay is also provided.

Method and compositions for enhancing aminolevulinic acid dehydratase

Detection of lead present in a sample, comprising the steps of: (a) adding a lead recovery agent to an assay solution containing lead from the sample; (b) adding to the assay solution a disulfide enzyme which is inhibited in the presence of lead; and (c) correlating the activity of the disulfide enzyme to the amount of lead in the sample. The lead recovery agent enhances the sensitivity and accuracy of the assay such that the assay can be readily automated for detection of lead in whole blood using commercially available automation systems.

Automated lead assay

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Spring Grove, IL, United States of America

David M Finley