Illumina Corporation

Arizona State University

Illumina Cambridge Limited

Singular Genomics Systems, Inc.

David Louis Heiner

Michal Lebl

Robert C Kain

Kevin L Gunderson

Chanfeng Zhao

Mark J Nibbe

Aaron Jones

Dale Buermann

Mark T Reed

Brett M Ellman

Steve R Burgett

Steven P Fambro

Matthew William Hage

Kamil Salloum

Bryan Crane

Jonathan Posner

Mark S Chee

Steven Mark Barnard

Alexandre Richez

Alexander Triener

John M Beierle

Sean M Ramirez

James M Tsay

Sang Ryul Park

Kevan Samiee

Peyton Shieh

Ludovic Vincent

Steve Fambro

Michel Perbost

Michael Aaron Schroeder

Anmiv Prabhu

Chad F Derosier

Scott William Bailey

Wei Wei

Tyler Jamison Dill

Robert Yang

Edwin Li

Theo Kotseroglou

Naiqian Zhan

Yuxiang Huang

Kristina Munoz

Leonid Malevanchik

Vit Pokorny

Samantha Schmitt

Sunmin Ahn

Valerie Uzzell

Eli N Glezer

David Marc Baranson

David L Barker

A technique for sequencing nucleic acids in an automated or semi-automated manner is disclosed. Sample arrays of a multitude of nucleic acid sites are processed in multiple cycles to add nucleotides to the material to be sequenced, detect the nucleotides added to sites, and to de-block the added nucleotides of blocking agents and tags used to identify the last added nucleotide. Multiple parameters of the system are monitored to enable diagnosis and correction of problems as they occur during sequencing of the samples. Quality control routines are run during sequencing to determine quality of samples, and quality of the data collected.

Independently removable nucleic acid sequencing system and method

A system for sequencing nucleic acid comprising a plurality of stations and a system control. The system control configured to direct the first substrate to progress and retrogress between the first processing station and the imaging station, direct the second substrate to progress and retrogress between the second processing station and the imaging station, and direct a chemistry cycle of a first sequencing procedure to occur within one of the first processing station or the second processing station while an imaging cycle of a second sequencing procedure occurs within the imaging station.

Nucleic acid sequencing system

Disclosed herein, inter alia, are imaging systems, devices, and methods of use thereof.

Kinematic imaging system

A flow cell package includes first and second surface-modified patterned wafers and a spacer layer. The first surface-modified patterned wafer includes first depressions separated by first interstitial regions, a first functionalized molecule bound to a first silane or silane derivative in at least some of the first depressions, and a first primer grafted to the first functionalized molecule in the at least some of the first depressions. The second surface-modified patterned wafer includes second depressions separated by second interstitial regions, a second functionalized molecule bound to a second silane or silane derivative in at least some of the second depressions, and a second primer grafted to the second functionalized molecule in the at least some of the second depressions. The spacer layer bonds at least some first interstitial regions to at least some second interstitial regions, and at least partially defines respective fluidic chambers of the flow cell package.

Flow cell package and method for making the same

Nucleic acid sequencing system and method

A method of fabricating a microarray including the steps of: (a) contacting a substrate having wells with a reagent reactive with said substrate to produce a surface modification within said wells and a surface modification surrounding said wells; (b) polishing said substrate to produce a polished surface modification surrounding said wells, wherein said surface modification surrounding said wells is removed and said surface modification within said wells is retained, and (c) depositing a biopolymer onto said substrate, wherein different affinities of said surface modification within said wells and said polished surface facilitate localization of said biopolymer within said wells.

Microfabrication methods for the optimal patterning of substrates

Disclosed herein are compositions, methods and systems for the processing of chemical reactions, such as the synthesis of polymers.

Methods and compositions for processing chemical reactions

A flow cell for use in a microfluidic detection system. The flow cell includes a flow cell body having a channel that extends along the flow cell body. The flow cell body has a substrate material that extends along the channel and that is transparent to at least one of excitation light or light emissions. The channel has a functionalized channel surface that includes reactive groups configured to attach to molecules for biochemical analysis. The flow cell also includes fluidic inlet and fluidic outlet ports provided on the flow cell body and an electroosmotic (EO) pump held within the flow cell body in fluid communication with the channel. The EO pump is operable to induce flow of a solution from the inlet port to the outlet port and along the functionalized channel surface.

Flow cells and manifolds having an electroosmotic pump

A system for sequencing nucleic acids, that includes (a) a table having an arrangement of sites, including a site for receiving a first substrate, and a site for receiving a second substrate, the substrates each having an array for providing sequencing data for a plurality of different nucleic acids in parallel; (b) a plurality of stations configured to carry out manipulations in a sequencing procedure; and (c) a system control interface configured to direct relative movement between the table and the plurality of stations, and to direct different steps of the sequencing procedure to occur at the sites for receiving the different substrates, wherein the first substrate can be removed from the system independently of the second substrate such that the second substrate can be processed to obtain sequencing data independently of the first substrate.

Growing community of inventors

San Diego, CA, United States of America

David Louis Heiner