General Electric Company

Leica Microsystems Cms Gmbh

Ge Healthcare Bio-sciences Corp.

Digital Pathco, LLC

David LaVan Henderson

Kevin Bernard Kenny

Robert John Filkins

Dashan Gao

Siavash Yazdanfar

Alex David Corwin

Brion Daryl Sarachan

Dirk Ryan Padfield

Michael Christopher Montalto

Christopher Reynolds Hammond

Stephen Eric Zingelewicz

Michael Adam Kinstrey

Xiaofeng Liu

Michael S Lazare

Helena Goldfarb

Richard R McKay

Stephen John Angelovich

Antti Seppo

Denise A Hollman-Hewgley

Mary Kathleen Callahan

Alexandra Jay Schmidt

John Espirito Santo Amaral

Ralph Andrew Minerva

Ying Li

Ainura Kyshtoobayeva

Natalia R Jun

Thomas Ha

Adriana Ines Larriera

Vipul A Baxi

A method for generating an image of a region of interest in a biological sample comprising the steps of: generating a first image including the region of interest of the biological sample having undergone a first protocol but not a second protocol; and generating a second image including the region of interest of the biological sample after having undergone a second protocol; wherein the region of interest is smaller than said sample. Also provided is a method of analyzing a biological sample, comprising providing an image of the biological sample according to the method for generating an image of a region of interest in a biological sample, and analyzing the biological sample from the image. Further provided are system and kit that comprise the means for executing the novel methods.

Methods for generating an image of a biological sample

Embodiments provide a user interface including an image panel for displaying a field of view corresponding to an image, or a portion of an image, of a biological specimen, and a biomarker panel for displaying thumbnail images for simultaneously viewing different aspects of a region within the field of view. Upon selection or update of the selected region of the field of view, the thumbnail images may be accordingly updated automatically to display the newly selected region. The image panel may include an interest region selection component for delineating a region within the field of view. The interest region selection component may be overlaid over a portion of the field of view displayed in the image panel, and may be used by a user to select or update the region within the field of view displayed in the biomarker panel. Images of interest may be saved to an electronic record.

Selection and display of biomarker expressions

In an imaging device having an objective and a stage for holding a sample to be imaged, a method for autofocusing is presented. The method includes determining a measured focus value corresponding to at least a first of a plurality of logical image segments. Further, the method includes imaging the first logical image segment using the measured focus value. The method also includes determining a predicted focus value for a second of the plurality of logical image segments using the measured focus value and a stored focus variation parameter. In addition, the method includes imaging the second logical image segment using the predicted focus value.

System and method for enhanced predictive autofocusing

Image quality is assessed for a digital image that is a composite of tiles or other image segments, especially focus accuracy for a microscopic pathology sample. An algorithm or combination of algorithms correlated to image quality is applied to pixel data at margins where adjacent image segments overlap and thus contain the same content in separately acquired images. The margins may be edges merged to join the image segments smoothly into a composite image, and typically occur on four sides of the image segments. The two versions of the same image content at each margin are processed by the quality algorithm, producing two assessment values. A sign and difference value are compared with other image segments, including by subsets selected for the orientation of the margins on sides on the image segments. The differences are mapped to displays. Selection criteria determine segments to be re-acquired.

Image quality assessment including comparison of overlapped margins

A method for imaging is presented. The method includes acquiring a plurality of images corresponding to at least one field of view using an imaging device having an objective and a stage for holding a sample to be imaged. Furthermore, the method includes determining a figure of merit corresponding to each pixel in each of the plurality of acquired images, wherein the figure of merit comprises a discrete approximation to a gradient vector. The method also includes synthesizing a composite image based upon the determined figures of merit.

System and method for imaging with enhanced depth of field

An Integration Controller integrates broadcast automation systems and thereby provides a single command and control center for monitoring production of the audio and video stream. An event-based schedule, or a &ldquo;playlist,&rdquo; specifies the segments to be played along with the necessary timing and hardware information. Each event in the schedule commands the playback or record of a video segment, generation of a special effect, transfer of a video segment from one device to another or closure of one or more video switches. Events may be launched automatically at a specified time, or manually by an operator. An Integration Controller (IC) communicates with an Event Scheduling System via a Scheduling System Interface. The Scheduling System Interface communicates with the Integration Controller via the IC server. The IC server utilizes various modules to communicate with Device Drivers, a Log Manager and one or more User Interfaces.

System and method for monitoring and controlling the production of audio and video streams

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David LaVan Henderson