University of Michigan

Kms Fusion, Inc.

Solohill Engineering, Inc.

David L Melmoth

David E Solomon

James Varani

William J Hillegas

An insert for a roller bottle of the type used in cell culture for vaccine manufacture is formed of a polymeric resilient material whereby it can be bent or rolled so that it can pass through the opening of the roller bottle. Once the insert is inside the bottle, it is released so that its resilient characteristic causes it to unbend or unroll. The insert is wider than the diameter of the roller bottle, and therefore it is urged against the interior wall of the roller bottle. Apertures and/or notches are provided in the insert to increase the turbulence of a fluid and to decrease the fluidic shear forces in the roller bottle as it is rotated in conventional operation. A significant increase in productivity is achieved because particles, which may be microcarrier beads, are maintained in suspension, and prevented from sinking to the bottom of the fluid medium where cells cannot grow. The roller bottle system is thereby transformed into a microcarrier suspension culture system.

Roller bottle system

An improved mount for an optical lens or similar device for achieving X-Y adjustment which includes four diametrically opposed 90.degree. spaced knife edges on a lens ring opposed on one side by a spring and on the other side by a micrometer adjustment to provide positive control in the X and Y directions, each motion being independent of the other.

Growing community of inventors

Ann Arbor, MI, United States of America

David L Melmoth