Tufts University

Massachusetts Institute of Technology

University of Illinois

University of Pennsylvania

United States of America As Represented by the Secretary of the Air Force

Columbia University

Northwestern University

The United States Government As Represented by the Department of Veterans Affairs

Other

University of Pittsburgh

Children's Medical Center Corporation

Boston University

Beth Israel Deaconess Medical Center, Inc.

Wake Forest University

Tulane Educational Fund

David L Kaplan

Fiorenzo G Omenetto

Xiaoqin Wang

Gary G Leisk

Brian D Lawrence

Hyoung-Joon Jin

Tim Jia-Ching Lo

Gordana Vunjak-Novakovic

Ung-Jin Kim

Lorenz Meinel

Jaehyung Park

Mark Cronin-Golomb

Benedetto Marelli

Jason Amsden

Tuna Yucel

John A Rogers

Rajesh R Naik

Cheryl Wong Po Foo

Hu Tao

Dae-Hyeong Kim

Jia Huang

Anne George

Michael L Lovett

Irene Georgakoudi

Noorjahan A Panjwani

Monica A Serban

Dana Cairns

Evangelia Bellas

Xianyan Wang

Chunmei Li

Benjamin P Partlow

Rina Nazarov

Jon Kluge

Jeffrey K Marchant

Lei Li

Brian Litt

Yonggang Huang

Gregory C Rutledge

Lindsay Wray

Jonathan Viventi

Sunghwan Kim

Konstantinos Tsioris

Sergey Fridrikh

Miaomiao Yang

Rina Sears

Biman B Mandal

Lee W Tien

Christopher M Cannizzaro

Eleanor M Pritchard

Cinzia Metallo

Charu P Vepari

Rak-Hwan Kim

Markus J Buehler

Amanda Murphy

Rodrigo R Jose

Shengjie Ling

Jaewon Choi

Onur Hasturk

Stephanie L McNamara

Alexander Nicholas Mitropoulos

Jin Woo Lee

James J Yoo

Gregory H Altman

Richard A Gross

Rebecca L Horan

Michele Muccini

Fang Deng

Eun Seok Gil

Stephen P McCarthy

David J Horan

Vijay Thomas John

Krishna Kumar

Luca Dal Negro

Kacey Gribbin Marra

Raffaella Capelli

J Peter Rubin

Stefano Toffanin

Martin Hunter

Mark A Brenckle

Noshir Pesika

Valentina Benfenati

Roberto Zamboni

Alfred L Allen

Gerard Kugel

Shenzhou Lu

Detlev Boison

Vittorio Montanari

Michael Levin

Carlos Estrada

Waseem K Raja

Siwei Zhao

Ahmed Ibrahim

Samuel Lin

Abdurrahman Abdurrob

Jelena Rnjak-Kovacina

Rosario Friedman

Sharad Gupta

Craig W Hanna

Hyeon Joo Kim

Compositions and methods for improved histological analysis of organoids are disclosed. The compositions and methods include silk-elastin-like polymers have temperature-induced shape change properties.

Organoids and methods of making and using the same

Tyramine-substituted silk fibroin compositions as well as methods for making and using the same are provided.

Chemical modification of silk fibroin for fabrication of enzymatically crosslinked hydrogels with tunable gelation kinetics and bioactivity

The present disclosure relates to compositions, methods of making, and methods of using a modified silk-based composition having a selectively tunable hydrophobicity. The provided compositions include silk fibroin having a haloalkyl substituent. The haloalkyl substituent is coupled to an amino acid of the silk fibroin though a linking agent.

Hydrophobic silk fibroin compositions and methods of making the same

In some embodiments, the present disclosure provides methods including the steps of (i) providing silk fibroin material comprising substantially amorphous structure, and (ii) applying at least one of elevated temperature and elevated pressure to the silk fibroin material to form a silk fibroin article, wherein the applying induces fusion between at least a portion of the silk fibroin and structural change of fibroin in the silk fibroin material. In some embodiments, the present disclosure also provides silk fibroin articles made in accordance with the methods disclosed herein.

Compression and heat-assisted production of silk-based materials

Saccharide-substituted silk fibroin compositions as well as methods for making and using the same are provided. The compositions can include at least one saccharide coupled to silk fibroin. The coupling can be via various residues on the silk fibroin.

Tunable silk-based biomaterials with saccharide substitutions, and methods of producing the same

Provided herein are methods and compositions for stabilization of active agents. The active agents are distributed, mixed or embedded in a silk fibroin matrix, thereby retaining the bioactivity of the active agents upon storage and/or transportation. In some embodiments, the storage-stable vaccine-silk compositions are also provided herein.

Compositions and methods for stabilization of active agents

A microneedle or microneedle device includes a microneedle body extending from a base to a penetrating tip formed from a silk fibroin based material, which is easy to fabricate and highly biocompatible. The microneedle device can include one or more microneedles mounted to a substrate. The silk fibroin can include active agents to be transported into or across biological barriers such as skin, tissue and cell membranes. The silk fibroin microneedles can be fully or partially biodegradable and/or bioerodible. The silk fibroin is highly stable, affords room temperature storage and is implantable. The silk fibroin structure can be modulated to control the rate of active agent delivery.

Silk fibroin-based microneedles and methods of making the same

The present application relates to silk fibroin-based hydrogels, methods for making and using the same.

Optically transparent silk hydrogels

The present disclosure provides transparent, elastic silk hydrogels for applications, including corneal reshaping to restore visual acuity and photolithography. The present disclosure also provides methods of photocrosslinking silk fibroin protein using riboflavin as a photoinitiator and exposing such riboflavin doped silk fibroin to light resulting in the formation of a transparent, elastic hydrogel.

Silk-fibroin hydrogels, methods of forming, and uses thereof

The present invention provides, among other things, methods for producing platelets including the steps of providing a silk membrane about 2 &#x3bc;m and 100 &#x3bc;m thick, inclusive, contacting the silk membrane with a porogen to form a porous silk membrane comprising at least one silk wall defining a lumen, associating the porous silk membrane with stromal derived factor-1? and at least one functionalizing agent, forming a three dimensional silk matrix comprising interconnected pores wherein the pores have a diameter of between about 5 and 500 &#x3bc;m, inclusive, wherein the silk matrix is formed around at least a portion of the porous silk membrane, introducing a plurality of megakaryocytes to the silk matrix such that the megakaryocytes are located at least partially within the porous silk matrix, and stimulating the plurality of megakaryocytes to produce platelets. Also provided are various new compositions and methods of making those compositions.

Microphysiologic methods and compositions

The inventions provided herein relate to compositions, methods, delivery devices and kits for repairing or augmenting a tissue in a subject. The compositions described herein can be injectable such that they can be placed in a tissue to be treated with a minimally-invasive procedure (e.g., by injection). In some embodiments, the composition described herein comprises a compressed silk fibroin matrix, which can expand upon injection into the tissue and retain its original expanded volume within the tissue for a period of time. The compositions can be used as a filler to replace a tissue void, e.g., for tissue repair and/or augmentation, or as a scaffold to support tissue regeneration and/or reconstruction. In some embodiments, the compositions described herein can be used for soft tissue repair or augmentation.

Injectable silk fibroin foams and uses thereof

The present invention, in some aspects, provides compositions including a solution comprising a plurality of exfoliated silk microfibrils and/or exfoliated silk nanofibrils, wherein the micro- or nano-fibrils are characterized as having a substantially nematic structure, as well as methods for making and using the same.

Silk nanofibrils and uses thereof

Provided herein relates to methods and compositions for preparing a silk microsphere and the resulting silk microsphere. In some embodiments, the methods and compositions described herein are all aqueous, which can be used for encapsulating an active agent in a silk microsphere, while maintaining activity of the active agent during processing. In some embodiments, the resulting silk microsphere can be used for sustained delivery of an active agent encapsulated therein.

Methods and compositions for preparing a silk microsphere

The present disclosure provides certain silk-fibroin compositions with particular characteristics and/or properties. In some embodiments, the disclosure provides low molecular weight compositions. In some embodiments, the disclosure provides silk fibroin compositions that comprise an active (e.g., a biological) agent or component. In some embodiments, the disclosure provides low molecular weight silk fibroin compositions that comprise an active (e.g., a biological) agent or component. In some embodiments, an active agent is stabilized in a silk composition, e.g., for a period of time and/or against certain conditions or events. In some embodiments, a component present in a silk fibroin composition may be subject to analysis and/or characterization. In some embodiments, a component present in a silk fibroin composition may be recovered from the composition.

Low molecular weight silk compositions and stabilizing silk compositions

The present invention provides, in some embodiments, multi-layer silk compositions including a first layer comprising silk fibroin and keratinocytes, a second layer comprising silk fibroin and fibroblasts, a third layer comprising silk fibroin and adipocytes, and a plurality of nervous system cells, wherein at least some of the plurality of nervous system cells span at least two layers, and methods of making and using the same. In some embodiments, provided methods and compositions further include immune cells and/or endothelial cells.

Innervated artificial skin

Growing community of inventors

Concord, MA, United States of America

David L Kaplan