Cognis Corporation

Other

Cognis IP Management Gmbh

David L Craft

C Ron Wilson

Krishna M Madduri

L Dudley Eirich

John C Loper

Maria Tang

Mark Eshoo

Cathy A Cornett

Alfred A Brenner

Martin Anthony Gleeson

Yeyan Zhang

Dudley Eirich

Jeffrey B Stavenhagen

A cummerbund belt assembly for retaining a cummerbund in a preferred position includes a cummerbund is wearable around a waist of a user to enhance the appearance of formal dress. A plurality of couplers is each coupled to and extends downwardly from the cummerbund. Each of the couplers is positioned at strategic locations along the cummerbund such that each of the couplers is aligned with a respective plurality of suspender buttons in the trousers. Moreover, each of the couplers releasably engages the respective suspender button to inhibit the cummerbund from riding upwardly on the user's waist when the cummerbund and the trousers are worn.

Cummerbund belt assembly

Genetically modified strains of, which will not revert to wild-type activity at the POX 4 and/or POX 5 locus, are disclosed. The strains are &#x3b2;-oxidation blocked and have been transformed through homologous recombination with a construct which deletes a portion of the POX 4 and/or POX 5 gene. The modified strains may be used to increase yields of dicarboxylic acids produced in host cells of the strains. Methods for blocking the &#x3b2;-oxidation pathway in ahost cell are also provided.

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The present invention provides modified promoters fromCYP and POX4 genes. The modified promoters have various sequence motifs added, deleted, or altered in order to modulate expression of a coding sequence operably linked thereto. The sequence motifs comprise repressors of gene induction (URS sequences) and activators of gene induction (UAS sequences) as well as oleic acid response elements (ORE sequences). Yeast host cells comprising such modified promoters are also provided. Methods of altering expression of a protein of the beta or omega oxidation pathways using a subject modified promoter are also provided.

The present invention provides fatty alcohol oxidase (FAO) proteins and nucleic acid molecules encoding the FAO proteins. Also provided are analogs, derivatives, and enzymatically active fragments of the FAO proteins. Vectors and host cells comprising the nucleic acid molecules encoding the FAO proteins, analogs, derivatives and enzymatically active fragments thereof are also provided. In addition, FAO signature peptides and isolated nucleic acid molecules encoding the signature peptides are provided by the present invention. Methods of producing or increasing production of a subject FAO protein, methods for increasing aldehyde production during the second step of the &#x3c9;-oxidation pathway of fatty acids, methods for increasing production of a ketone from an alcohol during the second step of the &#x3c9;-oxidation pathway of fatty acids, and methods for increasing production of a dicarboxylic acid are also provided.

Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzymes of the &#x3c9;-hydroxylase complex of20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.

A nucleic acid sequence including a CYP promoter operably linked to nucleic acid encoding a heterologous protein is provided to increase transcription of the nucleic acid. Expression vectors and host cells containing the nucleic acid sequence are also provided. The methods and compositions described herein are especially useful in the production of polycarboxylic acids by yeast cells.

Method of increasing conversion of a fatty acid to its corresponding dicarboxylic acid

Use of CYP52A2A promoter to increase gene expression in yeast

A novel gene has been isolated which encodes cytochrome b5 (CYTb5) protein of the &ohgr;-hydroxylase complex of 20336. Vectors including this gene, and transformed host cells are provided. Methods of increasing the production of a CYTb5 protein are also provided which involve transforming a host cell with a gene encoding this protein and culturing the cells. Methods of increasing the production of a dicarboxylic acid are also provided which involve increasing in the host cell the number of genes encoding this protein.

Cytochrome b5 gene and protein of Candida tropicalis and methods relating thereto

Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzymes of the &ohgr;-hydroxylase complex of,20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.

Cytochrome P450 monooxygenase and NADPH cytochrome P450 oxidoreductase genes and proteins related to the omega hydroxylase complex of Candida tropicalis and methods relating thereto

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Fort Thomas, KY, United States of America

David L Craft