Imperial Innovations Limited

Solexa, Inc.

Akubio Limited

Imperial College Innovations Limited

Inverness Medical Switzerland Gmbh

Ionscope Ltd

Illumina Cambridge Limited

David Klenerman

Victor Petrovich Ostanin

Yuri Evgenievich Korchev

Fedor Nikolaievich Dultsev

Shankar Balasubramanian

Chao Li

Andrew Shevchuk

Pavel Novak

Gregory Frolenkov

Richard Clarke

Colin Lloyd Barnes

Matthew Cooper

David Bentley

Christopher Abell

Max Joseph Lab

Mark Allen Osborne

Liming Ying

Andreas Bruckbauer

Yuri Engenievich Korchev

A method for interrogating a surface using scanning ion conductance microscopy (SICM), comprising the steps of:

Scanning ion conductance microscopy using surface roughness for probe movement

Scanning ion conductance microscopy using piezo actuators of different response times

The present invention is based on the realization that the bonds between a target molecule, or a target molecule attached to a particle, and a surface, can be ruptured by mechanically oscillating the surface at increasing amplitude, leading to detachment of the target molecule or particle from the surface. The required acceleration, and hence force, will depend on a variety of factors, including the mass of the molecule or particle, the nature of the bond to the surface and the geometric shape or size of the target molecule or particle. The present invention may therefore be used to separate or to size different target molecules, or to detect their presence.

Measurement and use of molecular interactions

The subject invention pertains to a method for the production of an array of molecules immobilised on a substrate, comprising the deposition of the molecules from a micropipette containing them, onto the substrate, in a liquid environment, wherein the distance of the micropipette from the substrate is controlled in response to the ion current in the liquid. This method is particularly suitable for the deposition of biological molecules. The subject invention also pertains to an array of biological molecules deposited on a substrate.

Production of molecular arrays

The invention is directed to a method for analysing genome wide variation in an individual. The method comprises randomly fragmenting the individual's genome and generating sequence reads of multiple bases on all fragments of the individual's genome, aligning the sequence reads generated with a known genomic reference sequence, and analysing variations between the sequence reads derived from the individual's genome and the known genomic reference sequence.

Arrayed biomolecules and their use in sequencing

The subject invention pertains to a method for determining the affinity between binding partners, or a property of one of the binding partners dependent on the affinity, comprising the steps of:

The present invention pertains to an apparatus for imaging an object, comprising a probe via which an assay component may be delivered; a sensor to detect ion current; and means for controlling the position of the probe relative to the object in response to the ion current. Such apparatus can be used to image live cells, without affecting them, in solution, e.g., using light, wherein the distance between probe and cell is less than the wavelength of light.

Optical microscopy and its use in the study of cells

A device comprising an array of molecules immobilised on a solid surface is disclosed, wherein the array has a surface density which allows each molecule to be individually resolved, e.g. by optical microscopy. Therefore, the arrays of the present invention consist of single molecules are more spatially distinct than the arrays of the prior art.

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Cambridge, United Kingdom

David Klenerman