Massachusetts Institute of Technology

Openmarket, Inc.

Soverain Software LLC

The Brigham and Women's Hospital, Inc.

Cisco Technology, Inc.

Harvard College

The Broad Institute, Inc.

Sightpath, Inc

David Kenneth Gifford

John R Ellis

G Winfield Treese

James W O'Toole, Jr

Richard Irving Sherwood

David R Liu

Daniela Rus

Hari Balakrishnan

Nisha Rajagopal

Max Walt Shen

Sejoon Lim

Mandana Arbab

Samuel R Madden

Jonathan Yee-Ting Hsu

The specification provides methods for introducing a desired genetic change in a nucleotide sequence using a double-strand break (DSB)-inducing genome editing system, the method comprising: identifying one or more available cut sites in a nucleotide sequence; analyzing the nucleotide sequence and available cut sites with a computational model to identify the optimal cut site for introducing the desired genetic change into the nucleotide sequence; and contacting the nucleotide sequence with a DSB-inducing genome editing system, thereby introducing the desired genetic change in the nucleotide sequence at the cut site.

Systems and methods for predicting repair outcomes in genetic engineering

Provided herein is an improved method for performing CRISPR/Cas based screening that is not dependent on viral cloning methods.

High-throughput CRISPR-based library screening

In some embodiments, a method of providing a user with a roadway route to a destination that maximizes the probability of reaching the destination by a deadline includes providing a database of traffic delay probability distributions based on historical traffic delay information, performing route-planning using at least one of the traffic delay probability distributions and parametric optimization to determine at least one route to the destination that maximizes the probability of reaching the destination by the deadline, and displaying the at least one route to the user. In some embodiments, the database includes a mean value and a variance value associated with each route from a plurality of routes stored at the database, and the performing includes the following steps: defining a first set of routes from the plurality of routes, selecting a first route from the plurality of routes as a current optimal route, defining a second set of routes and a third set of routes based on the first route, and discarding the second set of routes.

Method and apparatus for traffic-aware stochastic routing and navigation

Aspects of the invention include systems and methods for implementing security protocols. A server translates links from a protocol incompatible with a browser to a protocol compatible with the browser and back-translates the link. Another example aspect of the invention features systems and methods for managing authenticating credentials of a user. A server manages a user's authenticating credentials automatically on behalf of the user.

Managing transfers of information in a communications network

The invention features various techniques for managing transfers of information in public packet switched communications networks. One aspect of the invention features a system for extracting data from sources of network-based information in a communications network. An object embedding-program locates a script program and causes the script program to extract data from a page of information.

The invention features various techniques for managing transfers of information in public packet switched communications networks. In one aspect, the invention provides a system for identifying updated items of network-based information, such as pages, to users in a network. A master server receives the data from each of a plurality of network servers and merges them into one or more master logs. The logs have entries that pertaining to creation of or changing of pages of information. Another aspect of the invention features a system for implementing security protocols. A proxy server translates links from a protocol incompatible with the network tool to a protocol compatible with the network tool and back-translates the link. Another aspect of the invention features a system for managing authenticating credentials of a user. A proxy server manages a user's authenticating credentials automatically on behalf of the user. Another aspect of the invention features a system for inducing advertisers to target advertisements to consumers. An advertising broker receives advertisements and messages indicating that users. Have read the advertisements, and causes an offer having monetary value to be executed. Another aspect of the invention features a system for extracting data from sources of network-based information in a communications network. An object embedding-program locates a script program and causes the script program to extract data from a page of information.

Transfers of information in a communications network

The invention features various techniques for managing transfers of information in public packet switched communications networks. In one aspect, the invention provides a system for identifying updated items of network-based information, such as pages, to users in a network. A master server receives the data from each of a plurality of network servers and merges them into one or more master logs. The logs have entries that pertaining to creation of or changing of pages of information. Another aspect of the invention features a system for implementing security protocols. A proxy server translates links from a protocol incompatible with the network tool to a protocol compatible with the network tool and back-translates the link. Another aspect of the invention features a system for managing authenticating credentials of a user. A proxy server manages a user's authenticating credentials automatically on behalf of the user. Another aspect of the invention features a system for inducing advertisers to target advertisements to consumers. An advertising broker receives advertisements and messages indicating that users have read the advertisements, and causes an offer having monetary value to be executed. Another aspect of the invention features a system for extracting data from sources of network-based information in a communications network. An object embedding-program locates a script program and causes the script program to extract data from a page of information.

The present invention is a new method called replica routing that automatically directs client computers that request a service to a server replica for that service. The replica chosen by replica routing is the replica that is expected to provide the best performance to the client based upon the client's location in the internetwork topology and the estimated performance of the internetwork. In addition, the system and method is designed to permit new replicas to be flexibly added without undue administrative overhead.

Replica routing

The present invention relates to techniques for controlling transfers of information in computer networks. One technique involves transmitting from a server computer to a client computer a document containing a channel object corresponding to a communication service, and storing an access ticket that indicates that a user of the client computer permits the information source computer to communicate with the user over a specified channel. Another technique involves transmitting smart digital offers based on information such as coupons and purchasing histories stored at the computer receiving the offer. Another technique involves transmitting from a server computer to a client computer a request for a user's personal profile information, and activating a client avatar that compares the request for personal profile information with a security profile of the user limiting access to personal profile information. Another technique involves transmitting from a server computer to a client computer a document containing an embedded link, activating the embedded link at the client computer and recording activation of the embedded link in a metering log.

Controlled transfer of information in computer networks

Methods and products are provided for the oligonucleotide-mediated polymerase-dependent mutation of template nucleic acid sequences in a programmed sequence over multiple rounds of nucleic acid synthesis. The methods employ series of mutagenic primers, all of which may be present in the initial reaction system, in which at least some of the mutagenic primers do not bind to or promote polymerization from the original template nucleic acids, but do bind to and promote polymerization from the mutagenized products of earlier rounds of the program of sequential mutagenesis. The products of the invention include mixtures of such mutagenic primers. The products and methods have applications in the biological and computational sciences.

Programmed sequential mutagenesis

A complete system for the purchasing of goods or information over a computer network is presented. Merchant computers on the network maintain databases of digital advertisements that are accessed by buyer computers. In response to user inquiries, buyer computers retrieve and display digital advertisements from merchant computers. A digital advertisement can further include a program that is interpreted by a buyer's computer. The buyer computers include a means for a user to purchase the product described by a digital advertisement. If a user has not specified a means of payment at the time of purchase, it can be requested after a purchase transaction is initiated. A network payment system performs payment order authorization in a network with untrusted switching, transmission, and host components. Payment orders are backed by accounts in an external financial system network, and the payment system obtains account authorizations from this external network in real-time. Payment orders are signed with authenticators that can be based on any combination of a secret function of the payment order parameters, a single-use transaction identifier, or a specified network address.

Open network payment system for providing for real-time authorization of payment and purchase transactions

Digital active advertising

The present invention is a new method called replica routing that automatically directs client computers that request a service to a server replica for that service. The replica chosen by replica routing is the replica that is expected to provide the best performance to the client based upon the client's location in the internetwork topology and the estimated performance of the internetwork. In addition, the system and method is designed to permit new replicas to be flexibly added undue administrative overhead.

Growing community of inventors

Weston, MA, United States of America

David Kenneth Gifford