The Procter Gamble Company

Syneron Medical Ltd.

David John Weisgerber

Donn N Rubingh

Robert Wayne Glenn, Jr

Raymond Edward Bolich, Jr

Mark Leslie Kacher

James Charles Dunbar

Fernando Ray Tollens

James C Letton

Joseph J Elsen

Paul Elliott Correa

Timothy B Guffey

Robert Raymond Schmidt

Wayne Ellis Eccard

Jeffrey John Kester

Mannie Lee Clapp

Mary E Carethers

Christopher Dean Putman

Stephen A McCoy

Kevin Lee Hartzler

John R Baginski

Anna Reavis Husk

Andrew Campbell Allcock

Deborah Jean Back

Joseph McGrady

Bryan L Madison

James Anthony Letton

James B Hirshorn

Jeffrey K Kester

Peter M Self

Richard L Ingle

Nikki Elizabeth Annunziata

Tia Janinne Maurer

The present disclosure relates to subtilisin protease conjugate comprising a protease moiety and one or more addition moieties. Each addition moiety is covalently attached to an epitope protection position of the protease moiety. The protease conjugates have decreased immunogenicity relative to a parent protease. The present disclosure further relates to cleaning and personal care compositions comprising the protease conjugates.

Protease conjugates having sterically protected epitope regions

The present disclosure relates to subtilisin protease conjugate comprising a protease moiety and one or more addition moieties. Each addition moiety is covalently attached to a clip site protection position of the protease moiety, wherein the clip site protection positions are selected from 13, 14, 15, 16, 18, 19, 20, 21, 84, 85, 88, 158, 159, 160, 161, 162, 163, 164, 165, 170, 186, 191, 192, 193, 194, 196, 259, 260, 261, 262, and 274 corresponding to subtilisin BPN&prime;. The protease conjugates have decreased immunogenicity relative to a parent protease. The present disclosure further relates to cleaning and personal care compositions comprising the protease conjugates.

Protease conjugates having sterically protected clip sites

Protease conjugates such as subtilisin conjugates are provided comprising a protease moiety and one or more addition moieties wherein the protease moiety has a modified amino acid sequence of a parent amino acid sequence. The parent amino acid sequence comprises a first epitope region, a second epitope region and a third epitope region, and the modified amino acid sequence comprises a substitution by a substituting amino acid at one or more positions in one or more of the epitope regions. When a substitution occurs in the first epitope region, the substitution occurs at one or more positions corresponding to positions 70-84 of subtilisin BPN&prime;. When a substitution occurs in the second epitope region, the substitution occurs at one or more positions corresponding to positions 103-126 of subtilisin BPN&prime;. When a substitution occurs in the third epitope region, the substitution occurs at one or more positions corresponding to positions 217-252 of subtilisin BPN&prime;. Each addition moiety is covalently attached to one of the substituting amino acids. Cleaning and personal care compositions are provided containing the protease conjugates which have reduced immunogenicity relative to their corresponding parent proteases.

Proteases having modified amino acid sequences conjugated to addition moieties

The present invention relates to personal care compositions comprising a water insoluble substrate, a plurality of active proteins, and a binding means, comprising a polymeric tether, permanently attaching each of the enzymes to the substrate wherein the personal care composition comprises from about 0.01 &mgr;g/cm to about 1000 &mgr;g/cm of the enzyme on the substrate.

Personal care compositions containing active proteins tethered to a water insoluble substrate

The present invention relates to personal care compositions comprising a water insoluble substrate, a plurality of Protease G enzymes, and a binding means, permanently attaching each of the enzymes to the substrate wherein the personal care compositions comprise from about 0.01 &mgr;g/cm,to about 1000 &mgr;g/cm,of the enzyme on the substrate.

Personal care compositions containing subtilisin enzymes bound to water insoluble substrates

Modified polypeptides with high activity and reduced allergenicity

Disclosed is a stress stable lathering skin cleansing liquid composition comprising by weight parts of the liquid composition:

Crystalline hydroxy waxes as oil in water stabilizers for skin cleansing

The present invention relates to a stress stable lathering skin cleansing liquid composition comprising by weight parts of the liquid composition:

A stress stable lathering skin cleansing liquid composition comprising by weight parts of the liquid composition:

Reduced fat and calorie pourable shortenings, cooking oils, salad oils or similar compositions are disclosed. The nondigestible portion of these compositions contain low levels of solids, yet still exhibit good passive oil loss control. These compositions also have good temperature cycling stability and they impart good organoleptic properties to foods prepared with them. Also disclosed are relatively clear oils with reduced calories, All these pourable compositions comprise specific solid polyol fatty acid polyesters, a liquid nondigestible oil and, optionally, a digestible oil and/or a hardstock fat.

Reduced calorie pourable shortening, cooking oils, salad oils or like

Reduced fat and calorie plastic shortenings are disclosed. The nondigestible portion of these compositions contain low levels of solids, yet still exhibit good passive oil loss control. These compositions also impart good organoleptic properties to foods prepared with them. These shortenings comprise specific solid polyol fatty acid polyesters, a liquid nondigestible oil, and, optionally, a hardstock, a digestible oil, an intermediate melting triglyceride, or other shortening ingredients.

Shortening compositions containing polyol fatty acid polyesters

Fatty acid esters of polyols having at least 4 hydroxyl groups wherein the fatty acid groups consist essentially of: (1) long chain unsaturated fatty acid radicals containing at least 12 carbon atoms or mixtures of said unsaturated radicals with saturated fatty acid radicals containing 2 to 12 carbon atoms, and (2) long chain saturated fatty acid radicals containing at least 20 carbon atoms, wherein the molar ratio of (1):(2) is from 1:15 to 2:1 and wherein at least 4 of the hydroxyl groups of the polyol are esterified. The compounds are useful as nondigestible substitutes for solid fats in foods.

Solid, nondigestible, fat-like compounds and food compositions

Edible compositions comprising solid and liquid fatty materials, and having a high solids content, said solids having a particle size of 10 microns or less. Preferably, the fatty materials are wholly or partially non-digestible.

Fatty compositions with high solids content

The invention is a cocoa butter substitute comprising sucrose fatty acid esters having at least four fatty acid ester groups, and having the following fatty acid composition: (a) from about 25% to about 50% lauric acid; (b) from about 50% to about 75% palmitic acid; and (c) up to about 5% other fatty acids, preferably selected from the group consisting of capric, myristic, and stearic acids, and mixtures thereof. Alternatively, the cocoa butter substitute can comprise sucrose fatty acid esters having at least four fatty acid ester groups, and having the following fatty acid composition: (a) from about 25% to about 50% capric acid; (b) from about 50% to about 75% stearic acid; and (c) up to about 5% other fatty acids. The cocoa butter substitute is noncaloric and less expensive than natural cocoa butter. It has the same desirable melting properties as natural cocoa butter.

Sucrose polyesters which behave like cocoa butters

The invention is a process for post-hydrogenating sucrose polyesters with hydrogen gas in the presence of a catalytically effective amount of hydrogenation catalyst, at a temperature of from about 250.degree. F. (121.degree. C.) to about 450.degree. F. (232.degree. C.). Post-hydrogenation can be used to make sucrose polyesters that are different from those made by esterification. Preferably, the hydrogen pressure is at least about 20 psig. Increased hydrogen pressure overcomes steric hindrance of certain kinds of sucrose polyester molecules, allowing more complete hydrogenation of the fatty acids.

Growing community of inventors

Cincinnati, OH, United States of America

David John Weisgerber