Oxford Nanopore Technologies Limited

Oxford University Innovation Ltd

David Jackson Stoddart

James White

Andrew John Heron

James Anthony Clarke

John Hagan Pryce Bayley

Rebecca Victoria Bowen

Domenico Caprotti

Ramiz Iqbal Nathani

Paul Richard Moody

Richard Muscat

Lakmal Nishantha Jayasinghe

Christopher James Wright

Mark Bruce

Giovanni Maglia

Clive Gavin Brown

Daniel John Turner

Stuart William Reid

Jessica Mary May Johnson

Mark John Bruce

Lakmal Jayasinghe

Jessica Mary May Knott

METHODS AND SYSTEMS FOR CHARACTERIZING ANALYTES USING NANOPORES

The invention relates to a method for sequencing a heteropolymeric target nucleic acid sequence that involves stochastic sensing. The invention also relates to a method for improving a pore for sequencing a target nucleic acid sequence by modifying one or more sites in the pore.

Method for sequencing a heteropolymeric target nucleic acid sequence

Methods of characterizing an analyte using a detector such as a nanopore and an enzyme are provided. One aspect features methods for characterizing a double-stranded polynucleotide using a detector, e.g., without using a hairpin connecting a template and a complement of the double-stranded polynucleotide. Another aspect features methods for characterizing an analyte using a tag-modified nanopore with increased sensitivity and/or higher throughput. Compositions and systems including, e.g., adaptors for attachment to double-stranded polynucleotides and tag-modified nanopores, which can be used in the methods are also provided.

Method of characterizing a polynucleotide

The invention relates to a method for modifying a template double stranded polynucleotide, especially for characterisation using nanopore sequencing. The method produces from the template a plurality of modified double stranded polynucleotides. These modified polynucleotides can then be characterised.

Method for modifying a template double stranded polynucleotide

Methods of characterizing an analyte using a nanopore. One aspect features methods for characterizing a double-stranded polynucleotide using a nanopore, e.g., without using a hairpin connecting a template and a complement of the double-stranded polynucleotide. Another aspect features methods for characterizing an analyte using a tag-modified nanopore with increased sensitivity and/or higher throughput. Compositions and systems including, e.g., adaptors for attachment to double-stranded polynucleotides and tag-modified nanopores, which can be used in the methods are also provided.

Methods and systems for characterizing analytes using nanopores

Polynucleotide modification methods

The invention relates to a method for modifying a template double stranded polynucleotide, especially for characterization using nanopore sequencing. The method produces from the template a plurality of modified double stranded polynucleotides. These modified polynucleotides can then be characterized.

The invention relates to a method for modifying a template polynucleotide for characterization, especially for nanopore sequencing. The method produces a modified polynucleotide which is complementary to the template polynucleotide at some positions and which contains universal or abasic nucleotides at the other, and in some instances predicable, positions. The resulting modified polynucleotide can then be characterized.

Sample preparation method

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Oxford, United Kingdom

David Jackson Stoddart