Calgene Corporation

David J Schaaf

David M Stalker

Guangning Ye

Narender S Nehra

Kevin E McBride

Michael E Himmel

Jeffrey M Staub

Peter T Hajdukiewicz

Vladimir Sidorov

Steven R Thomas

A method is provided for transforming solanaceous plants to express DNA sequences interest from the plant cell plastid. The improved method allows the transformation of solanaceous plant tissue which is not obtained from tobacco with DNA constructs. Such DNA constructs comprise, in the 5&prime; to 3&prime; direction of transcription, a promoter region functional in a plant plastid and a DNA sequence of interest. The method can be utilized in the transformation of solanaceous plants, such as potato and petunia. The invention further provides constructs and methods for the expression of green fluorescent protein from the plant cell plastid.

Plastid transformation of solanaceous plants

Provided are constructs and methods for expressing herbicide tolerance genes in plastids of plant cells. Constructs include the components of a promoter functional in a plant plastid, a DNA sequence which is capable of conferring tolerance in a plant cell to at least one herbicide compound when said DNA sequence is transcribed in plastids of said plant cell and a transcription termination region. Herbicide tolerance is produced by transforming plastids with the constructs of the invention and growing plant cells comprising the transformed plastids under conditions wherein the DNA sequence is transcribed and plant plastids and cells containing the plastids are rendered tolerant to applications of at least one herbicide compound.

Expression of herbicide tolerance genes in plant plastids

Novel compositions and methods useful for genetic engineering of plant cells to provide expression in the plastids of a plant or plant cell of cellulose degrading enzymes.

Expression of enzymes involved in cellulose modification

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Davis, CA, United States of America

David J Schaaf