Pioneer Hi-bred International, Inc.

Babcock & Wilcox Power Generation Group, Inc.

Air Products and Chemicals, Inc.

Other

E.i. Dupont De Nemours and Company

Mcdermott Technology, Inc.

Pioneer Hi-bred Int'l Inc.

Prodigene, Inc.

Derrick Witcher

James C., Iii Register

A. Gururaj Rao

John Howard

Terry Euclaire Meyer

Chris Baszycynski

Thomas Czapla

David J Peterson

Benjamin A Bowen

Laura Ann Tagliani

Christopher L Baszczynski

William James Gordon-Kamm

Keith S Lowe

Christopher Jay Scelonge

Grace Marie St Clair

Zuo-Yu Zhao

Gary A Sandahl

Bruce J Drummond

Bing-Bing Wang

Ronald Dureiko

Michael Francis Carolan

Tong Zhu

Joshua K Young

Merrill Anderson Wilson

Sergei Svitashev

Kurt E Kneidel

Ronald B Johnson

Christopher M Chen

Keith Gerard Rackers

Stephen Lawrence Gasior

Kathryn Beverly Dyer, Legal Representative

John H Duesing

J Jeffrey Kidwell

Saverio Carl Falco

Michael W Lassner

Andrew Mark Cigan

Zhongsen Li

John A Howard

Wesley B Bruce

Zhan-Bin Liu

Huirong Gao

Derrick Ryan Witcher

Leszek Aleksander Lyznik

Elizabeth E Hood

Thomas H Czapla

James C Register, Iii

Paul John Berbakov

Ted Ohrn

Ted R Ohm

Daniel H Gibson

Christopher L Baszcynski

Grace M St Clair

Gary W Diefendorff

Bruce P Guilliams

A Gururaj Rao

Iii Register James C

Elizabeth Hood

Methods and compositions are provided for the improvement of double-strand-break-inducing agent activity in eukaryotic cells, through the usage of one or more morphogenic factors or developmental genes. The morphogenic factor may be provided to the same cell or to a different cell than that comprising or receiving the double-strand-break-inducing agent. The morphogenic factor may be provided to a cell as a polynucleotide composition on a recombinant vector, and may be placed on the vector outside of a T-DNA border. The morphogenic factor may be provided via an upregulation of an endogenous gene. The morphogenic factor, or the double-strand-break-inducing agent, may further comprise a cell penetrating peptide. The morphogenic factor may be co-introduced with a vector comprising RepA.

Use of morphogenic factors for the improvement of gene editing

A variety of methods and compositions are provided, including methods and compositions for targeted modification of a specific target site in a cell or organism, methods for integrating polynucleotides of interest, methods to assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, silence a gene, and identify and/or characterize transcriptional regulating regions. The methods involve the introduction of a cell proliferation factor and a double-strand break-inducing enzyme into an organism.

Methods and compositions for targeted polynucleotide modification

Compositions and methods are provided for the targeted integration of a polynucleotide sequence of interest into the genome of a plant or plant cell. The methods and compositions employ recognition sites for endonucleases and endonucleases in combination with site-specific recombination sites/recombinases to provide an effective system for establishing target sites within the genome of a plant, plant cell or seed. Once such target sites are established, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest.

Methods and compositions for targeted integration in a plant

A variety of methods and compostions are provided, including methods and compositions for targeted modification of a specific target site in a cell or organism, methods for integrating polynucleotides of interest, methods to assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excize a polynucleotide, silence a gene, and identify and/or characterize transcriptional regulating regions. The methods involve the introduction of a cell proliferation factor and a double-strand break-inducing enzyme into an organism.

Compositions for the targeted integration of nucleotide sequences into a plant are provided. Plants and plant cells having multiple transfer cassettes stacked at precise locations in the genome of a plant or plant cell are provided. In specific embodiments, the plant or plant cell comprises at least two transfer cassettes. The first transfer cassette comprises a first nucleotide sequence of interest flanked by a first and a second recombination site, wherein the first and the second recombination sites are non-identical. The second transfer cassette comprises a second nucleotide sequence of interest flanked by the second recombination site and a third recombination site, wherein the third recombination site is non-identical to the first and said second recombination site. The second recombination site is shared between the first and the second transfer cassette, such that the genome of the plant cell comprises in the following order the first recombination site, the first nucleotide sequence of interest, the second recombination site, the second nucleotide sequence of interest, and the third recombination site.

Compositions for the targeted insertion of a nucleotide sequence of interest into the genome of a plant

An improved wet scrubber tray provides increased resistance to vibration-induced cracking. The self-supporting tray eliminates wastage of raw material, and additional internal stiffeners added to the tray make multiple intermediate stiffeners. The connection of the intermediate stiffeners to the perforated bottom plate is changed to eliminate the shop weld in the bottom plate. The holes in the perforated bottom plate are moved away from the boundaries of the tray to reduce their influence on boundary conditions. Trays are held down by plate material that is attached to the support grid, but not to the trays. Three field welds are applied to the sides of each tray to hold the trays in place relative to each other and are strategically placed to allow adjacent side stiffeners to act together as a composite section. Elastomeric material is used at the tray-to-grid attachments increases the damping of the system.

Wet scrubber tray

Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.

Compositions and methods for the targeted insertion of a nucleotide sequence of interest into the genome of a plant

A variety of methods and compostions are provided, including methods and compositions for targeted modification of a specific target site in a cell or organism, methods for integrating polynucleotides of interest, methods to assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, silence a gene, and identify and/or characterize transcriptional regulating regions. The methods involve the introduction of a cell proliferation factor and a double-strand break-inducing enzyme into an organism.

Compositions and methods for genetic modification of plants

An improved wet scrubber tray provides increased resistance to vibration-induced cracking. The tray is self supporting to eliminate wastage of raw material, and additional internal stiffeners added to the tray make multiple intermediate stiffeners. The connection of the intermediate stiffeners to the perforated bottom plate is changed to eliminate the shop weld in the bottom plate. The holes in the perforated bottom plate are repositioned so that they are moved away from the boundaries of the tray to reduce their influence on boundary conditions. The thickness of the tray material may be increased to lower the vibratory stresses. The mechanism for holding the trays themselves within the spray absorber tower is changed so that the trays are now held down by plate material that is attached to the support grid, but not to the trays. Three field welds are applied to the sides of each tray to hold the trays in place relative to each other and are strategically placed to allow adjacent side stiffeners to act together as a composite section, thus increasing their strength. Elastomeric material such as neoprene is used at the tray-to-grid attachments to increase the damping of the system. Formed features in the trays such as single or multiple ribs, bosses, domes or dimples may also be provided to increase tray stiffness or influence wet scrubber performance.

Methods and compositions for the stacking of multiple nucleotide sequences at precise locations in the genome of a plant or plant cell are provided. Specifically, transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are introduced into a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase. The transfer cassettes and target sites are designed so as to allow for the stacking or ordering of nucleotide sequences at precise locations in the plant genome.

Compositions and methods to stack multiple nucleotide sequences of interest in the genome of a plant

An assembly and method for facilitating the attachment of a metal alloy hood to the concrete shell of a spray absorber tower, and compensating for relative radial thermal expansion of the hood and the shell during start up, shut down, and operation of the spray absorber tower, while also providing accurate and effective distribution of shear forces circumferentially along a horizontal plane from the metal alloy hood to anchor rods partially embedded in the concrete shell of the spray absorber tower.

Absorber tower metal hood to concrete shell attachment

Methods to find optimal integration sites within a plant genome are provided. More particularly, a plant is transformed with a target site having an expression cassette comprising a nucleotide sequence operably linked to a promoter active in the plant. The target site is flanked by non-identical recombination sites. Transformed protoplast, tissues, or whole plants can be tested to determine the levels of activity of the inserted gene. By comparison of cellular activities of the gene in different insertion sites, preferred integration sites may be found wherein the gene is expressed at high or acceptable levels. These plants can then be utilized with subsequent retargeting techniques to replace the nucleotide sequence with other genes or nucleotide sequences of interest contained in a transfer cassette.

Compositions and methods for locating preferred integration sites within the genome of a plant

Growing community of inventors

Ames, IA, United States of America

David J Peterson