E.i. Dupont De Nemours and Company

Butamax Advanced Biofuels LLC

Butamax (tm) Advanced Biofuels LLC

David J Lowe

Antoinette E Wilkins

John P O'Brien

Hong Peng Wang

Scott D Cunningham

Arie Ben-Bassat

Joseph J Zaher

Brian Michael Roesch

Michael Charles Grady

Keith H Burlew

Lisa L Huang

John W Hallam

Sharon Loretta Haynie

Fateme Sima Sariaslani

Raymond Richard Zolandz

Ranjan Patnaik

James Timothy Cronin

Malcolm S Smith

Benjamin Fuchs

Richard James Powell

Stephane Francois Bazzana

Clare A Stewart

Adam Bernfeld

Mathias E Stolarski

James Gregory Wood

Roy Jackson

Duncan Coffey

Stephan E Francois Bazzana

Mark Lazar

Michele Rb Malinowski

Bruce M Bakken

Adam Jones

A method and system for efficiently producing a fermentative product alcohol such as butanol utilizing in situ product extraction are provided. The efficiency is obtained through separating undissolved solids after liquefying a given feedstock to create a feedstock and prior to fermentation, for example, through centrifugation. Removal of the undissolved solids avoids problems associated with having the undissolved solids present during in situ production extraction, and thereby increases the efficiency of the alcohol production.

Methods and systems for removing undissolved solids prior to extractive fermentation in the production of butanol

The present invention relates to the fermentative production of alcohols including ethanol and butanol, and processes for improving alcohol fermentation employing in situ product removal methods.

Processes and systems for the fermentative production of alcohols

Peptide-based organic sunscreens, formed by coupling a skin-binding peptide with an organic sunscreen agent, are described. The skin-binding peptide part of the peptide-based organic sunscreen binds strongly to the skin, thus keeping the organic sunscreen agent attached to the skin for long lasting protection. Sunscreen compositions comprising the peptide-based organic sunscreens are also provided.

Peptide-based organic sunscreens

Methods for the microbial production of para-hydroxycinnamic acid (pHCA) and cinnamic acid (CA) are provided. Microbes producing either tyrosine or phenylalanine are grown in the presence of either tyrosine ammonium lyase or phenylalanine ammonium lyase respectively where some part of the fermentation is accomplished at alkaline pH. The process results in greater yields and higher rates of para-hydroxycinnamic acid (pHCA) and cinnamic acid (CA) production as compared with fermentation exclusively at physiological pH.

Methods for the preparation of para-hydroxycinnamic acid and cinnamic acid at alkaline pH

Combinatorially generated peptides are provided that have binding affinity for polypropylene (PP). The peptides may be used to deliver benefit agents to various PP surfaces.

Polypropylene binding peptides and methods of use

Combinatorially generated peptides are provided that have binding affinity for polyethylene (PE). The peptides may be used to deliver benefit agents to various PE surfaces.

Polyethylene binding peptides and methods of use

Combinatorially generated peptides are provided that have binding affinity for polymethylmethacrylate (PMMA). The peptides may be used to deliver benefit agents to various PMMA surfaces.

PMMA binding peptides and methods of use

Combinatorially generated peptides are provided that have binding affinity for nylon (NY). The peptides may be used to deliver benefit agents to various NY surfaces.

Nylon binding peptides and methods of use

Combinatorially generated peptides are provided that have binding affinity for Polytetrafluoroethylene (PTFE). The peptides may be used to deliver benefit agents to various PTFE surfaces.

Polytetrafluoroethylene binding peptides and methods of use

Combinatorially generated peptides are provided that have binding affinity for polystyrene (PS). The peptides may be used to deliver benefit agents to various PS surfaces.

Polystyrene binding peptides and methods of use

A biocatalytic method for preparing para-hydroxystyrene from para-hydroxycinnamic acid is described. The method uses an enzyme source having para-hydroxycinnamic acid decarboxylase activity to catalyze the decarboxylation of para-hydroxycinnamic acid in a biphasic reaction medium to produce para-hydroxystyrene, which is extracted into the organic phase of the biphasic reaction medium. The method results in a high yield of para-hydroxystyrene due to the decreased exposure of the enzyme source to the inhibitory product. The product is readily recovered from the extractant, or may be chemically derivatized directly in the extractant before recovery.

Method for preparing para-hydroxystyrene by biocatalytic decarboxylation of para-hydroxycinnamic acid in a biphasic reaction medium

This invention relates to a bioprocess engineering solution for a product removal process for use in a biofermentation. The invention discloses a process for withdrawing an aliquot of broth from a biofermentation vessel during at least a portion of the biofermentation, removing biocatalyst and water, chromatographically separating biofermentation products from the withdrawn broth using water as an eluent, and returning the remaining components of the broth back to the biofermentation vessel. Process chromatography permits highly selective separation of the target molecule, preventing feedback inhibition of the biofermentation.

Product removal process for use in a biofermentation system

Methods are provided for the production and recovery of multifunctional aromatic compounds from a fermentation medium. Preferred multifunctional aromatic compounds include para-hydroxycinnamic acid (pHCA), cinnamic acid (CA), and para-hydroxystyrene (pHS). The multifunctional aromatic compounds may be produced in a biphasic growth medium comprising a fermentation medium having a specified volume of an extractant. The multifunctional aromatic compounds are extracted into the extractant and recovered by standard means.

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Wilmington, DE, United States of America

David J Lowe