Ranpak Corporation

Guild Associates, Inc.

Other

Battelle Memorial Institute

Case Western Reserve University

Battelle Development Corporation

David J Fink

Kenneth E Hughes

Richard S Brody

David C Masterson

Barbara A Metz

Gordon E Pickett

Paul M Gemmer

Kevin M Virnelson

Thomas J Ward

Arnold I Caplan

Salvatore T DiNovo

Sudhakar Kadiyala

Randell G Young

Randall G Young

Joel C Preston

Timothy B Hutson

An implant for repair of a tissue defect comprises a plurality of physiologically compatible load-bearing sutures for securing under tension tissue adjacent to the defect to be repaired, the sutures for supporting a tissue reparative cell mass in the defect and a tissue reparative cell mass supported thereby. The sutures have a central portion encapsulated in a cell containing matrix which is contracted under a tensile load by the cells thereof and formed into a mat sheet during the contraction. Spring metal wires hold the sutures in tension during the contraction. The matrix is a collagen gel or other material which the cells contract, the cells comprising human mesenchymal stem cells. The mat sheet is then rolled into a spiral roll with the sutures extending from opposite roll ends to form the desired implant. Different embodiments are disclosed.

Biomatrix for soft tissue regeneration using mesenchymal stem cells

An implant for repair of a tissue defect which implant comprises a physiologically compatible load-bearing member having an element for securing under tension tissue adjacent to the defect to be repaired, an element for supporting a tissue reparative cell mass in the defect and a tissue reparative cell mass supported thereby. The implant can be a suture material having a cell containing matrix surrounding a central portion thereof. The matrix is preferably a gel or other material which the cells cause to contract, thereby drawing together the tissues surrounding the defect to which the implant is attached.

Biomatrix for soft tissue regeneration

A method for making a collagen strengthened cellulosic sheet by providing a cellulosic pulp slurry; adding solubilized collagen to the pulp slurry, and mixing for a time effective for interaction of the cellulosic pulp slurry and solubilized collagen; forming the interacted cellulosic pulp slurry and solubilized collagen into a sheet; and drying the sheet; also, a method for using solubilized collagen for strengthening paper by mixing the solubilized collagen with a cellulosic pulp slurry; and making a cellulosic pulp product from the mixture and drying.

Paper strengthened with solubilized collagen and method

The invention includes a method that provides a low cost aqueous solution of solubilized collagen by the steps of: (a) providing an aqueous ground slurry of insoluble collagen and adjusting the pH of said slurry to obtain activity for a proteolytic enzyme added in Step b; (b) adding said proteolytic enzyme to said pH adjusted slurry; (c) reacting the slurry and enzyme of Step b and/or recycled insoluble collagen and enzyme from Step e at a temperature, T, and for a time, t, effective for forming a solution increased in solubilized collagen; (d) adding additional water and insoluble collagen to said solution of Step c and mixing; (e) separating at least some of the solution of Step d containing solubilized collagen from insoluble collagen, whereby at least a portion of said insoluble collagen and proteolytic enzyme is recycled to Step c, and the separated solution containing solubilized collagen is withdrawn as product; an alternative embodiment provides for the direct production of solubilized collagen without the recycle step.

Recycle process for the production of low-cost soluble collagen

The invention includes an improved method for producing an aqueous solution of soluble collagen. The method comprises the steps of:

Method for the production of soluble collagen

Method of preparing paper strengthened with solubilized collagen

A preparing of a self-supporting or free-standing three-dimensional unitary structural body by a method including generating successively a plurality of cross-sectional layers of the body, one layer top of the other and with the layers joined together to form the body and with the generating of a cross-sectional layer comprising placing a plurality of drops of a liquid composition, containing a first reactant, in a pattern of discrete drops making up the cross-sectional layer and subsequently placing a plurality of discrete drops of other liquid composition, containing another reactant, in contact with the placed drops ion the pattern so that the first reactant and the other reactant react to provide a solid.

Method for free-formation of a free-standing, three-dimensional body

The invention includes a method that produces a low cost aqueous solution of solubilized collagen by the steps of: (a) providing an aqueous ground slurry of insoluble collagen and adjusting the pH of said slurry to obtain activity for a proteolytic enzyme added in Step b; (b) adding said proteolytic enzyme to said pH adjusted slurry; (c) reacting the slurry and enzyme of Step b and/or recycled insoluble collagen and enzyme from Step e at a temperature, T, and for a time, t, effective for forming a solution increased in solubilized collagen; (d) adding additional water and insoluble collagen to said solution of Step c and mixing; (e) separating at least some of the solution of Step d containing solubilized collagen from insoluble collagen, whereby at least a portion of said insoluble collagen and proteolytic enzyme is recycled to Step c, and the separated solution containing solubilized collagen is withdrawn as product; an alternative embodiment provides for the direct production of solubilized collagen without the recycle step.

Growing community of inventors

Shaker Heights, OH, United States of America

David J Fink