Isis Pharmaceuticals, Inc.

Ibis Biosciences, Inc.

Other

David J Ecker

Rangarajan Sampath

Steven A Hofstadler

Stanley T Crooke

Richard Holmes Griffey

John McNeil

Thomas A Hall

Lawrence B Blyn

Phillip Dan Cook

Christopher K Mirabelli

Mark W Eshoo

Timothy A Vickers

Susan M Freier

Jacqueline R Wyatt

Peter W Davis

Oscar L Acevedo

Brett P Monia

Lex M Cowsert

James C Hannis

Thomas W Bruice

Kevin P Anderson

Ronnie C Hanecak

Gregory E Hardee

Vivek Samant

Kenneth G Draper

Jared James Drader

Normand Hebert

Vickie L Brown-Driver

Yun Jiang

Neill K White

Muthiah Manoharan

C Frank Bennett

John S Kiely

Michael Egholm

Peter Eigil Nielsen

Yogesh S Sanghvi

Rolf Henrik Berg

Ole Buchardt

Lloyd G Tillman

Ching-Leou Teng

Niels E Mollegaard

Raymond Ranken

Eric E Swayze

Clarence Frank Bennett

Rahul C Mehta

Kristin Sannes Lowery

Ming-Yi Chiang

Yali Jennifer Tsai

Michael V Templin

Jean Louis Imbach

Ray Ranken

Christian Massire

Ranga Sampath

Graham Davis

Brenda F Baker

Andrew Mamoru Kawasaki

Roland Stoughton

Mark A Hayden

Gordon Bruce Collier

Charles John Guinosso

Walter F Lima

Jeffrey B Huff

Venkatraman Mohan

Glenn D Hoke

Kandasamy Ramasamy

Dan Wang

Stanley T Motley

Lendell Cummins

Kristin Sannes-Lowery

Duane J Knize

Todd P Michael

Debra L Robertson

Tim Vickers

Pete Davis

Danny M Chou

Stephen A Hofstadler

Dino J Sofianos

Karl H Rudnick

Cecil L Basham, Jr

Clifford T Lewis

Brons M Larson

Jonathan R Bar-on

Dennis P Murray

David W Robbins

John P Penhune

Normand Herbert

Neil White

The present invention compositions, methods and systems to identify, detect, and/or quantify bacterial DNA in the presence of contaminating non-bacterial DNA. In particular, the present invention provides oligonucleotides configured to detect a relatively small amount of bacterial DNA in the presence of an overwhelmingly large amount of contaminating human DNA.

Bioagent detection oligonucleotides

The present invention provides methods for rapid forensic analysis of mitochondrial DNA and methods for characterizing heteroplasmy of mitochondrial DNA, which can be used to assess the progression of mitochondrial diseases.

Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy

The present invention provides methods of: identifying pathogens in biological samples from humans and animals, resolving a plurality of etiologic agents present in samples obtained from humans and animals, determining detailed genetic information about such pathogens or etiologic agents, and rapid detection and identification of bioagents from environmental, clinical or other samples.

Methods for rapid identification of pathogens in humans and animals

Provided herein is technology relating to sequencing nucleic acids and particularly, but not exclusively, to devices, methods, and systems for sequencing-by-synthesis using changes in pH to monitor base addition. In some embodiments the electrochemical hydrogen ion sensor is a microfabricated mixed metal oxide electrode and in some embodiments the electrochemical hydrogen ion sensor is a membrane electrode. Moreover, in some embodiments the device further comprises a reference electrode. Performing the sequencing reaction involves moving solutions and other fluids (e.g., samples, nucleotide solutions, wash solutions) into and out of the reaction vessel. Thus, in some embodiments, the device further comprises a tube or other transport mechanism or pathway attached to the reaction vessel.

Nucleic acid sequencing by electrochemical detection

Provided herein are compositions and methods for the alteration of neuronal methylation by synthetic piRNAs or by alteration of piRNA function. Such alterations find use in the regulation and control of neural gene expression and concomitant neural functions. Further provided herein are systems and methods for the identification of target sites for regulation by piRNAs.

Alteration of neuronal gene expression by synthetic piRNAs and by alteration of piRNA function

The present invention provides methods for the quantification of an unknown bioagent in a sample by amplification of nucleic acid of the bioagent, and concurrent amplification of a known quantity of a calibration polynucleotide from which are obtained a bioagent identifying amplicon and a calibration amplicon. Upon molecular mass analysis, mass and abundance data are obtained. The identity of the bioagent is then determined from the molecular mass of the bioagent identifying amplicon and the quantity of the identified bioagent in the sample is determined from the abundance data of the bioagent identifying amplicon and the abundance data of the calibration amplicon.

Methods for concurrent identification and quantification of an unknown bioagent

The present invention relates to portable systems and devices, and corresponding methods, for detecting bioagents. In particular, the present invention provides systems, devices, and methods that utilize one or more of a sample preparation component, sample analysis component employing broad range primers, and sample detection component.

Bioagent detection systems, devices, and methods

The methods disclosed herein relate to methods and compositions for amplifying nucleic acid sequences, more specifically, from nucleic acid sequences of pathogens by targeted whole genome amplification.

Targeted whole genome amplification method for identification of pathogens

The present invention relates generally to the field of investigational bioinformatics and more particularly to secondary structure defining databases. The present invention further relates to methods for interrogating a database as a source of molecular masses of known bioagents for comparing against the molecular mass of an unknown or selected bioagent to determine either the identity of the selected bioagent, and/or to determine the origin of the selected bioagent. The identification of the bioagent is important for determining a proper course of treatment and/or irradication of the bioagent in such cases as biological warfare. Furthermore, the determination of the geographic origin of a selected bioagent will facilitate the identification of potential criminal identity.

Secondary structure defining database and methods for determining identity and geographic origin of an unknown bioagent thereby

The present invention provides methods for rapid forensic investigations by identification of bioagents associated with biowarfare and acts of terrorism or crime. The methods are also useful for epidemiological investigations by genotyping of bioagents.

Method for rapid detection and identification of bioagents in epidemiological and forensic investigations

The present invention relates to compositions and methods which enhance the local and systemic uptake and delivery of oligonucleotides and nucleic acids via non-parenteral routes of administration. Pharmaceutical compositions comprising oligonucleotides disclosed herein include, for systemic delivery, emulsion and microemulsion formulations for a variety of applications and oral dosage formulations. It has also surprisingly been discovered that oligonucleotides may be locally delivered to colonic sites by rectal enemas and suppositories in simple solutions, e.g., neat or in saline. Such pharmaceutical compositions of oligonucleotides may further include one or more penetration enhancers for the transport of oligonucleotides and other nucleic acids across mucosal membranes. The compositions and methods of the invention are utilized to effect the oral, buccal, rectal or vaginal administration of an antisense oligonucleotide to an animal in order to modulate the expression of a gene in the animal for investigative, therapeutic, palliative or prophylactic purposes.

Compositions and methods for non-parenteral delivery of oligonucleotides

Growing community of inventors

Encinitas, CA, United States of America

David J Ecker