Koninklijke Philips Corporation N.v.

Koninklike Philips N.v.

David Halter

Reinhold Wimberger-Friedl

Mark Thomas Johnson

Christiaan Varekamp

Johannes Hendrikus Maria Spruit

Emiel Peeters

Eduard Gerard Marie Pelssers

Marco Baragona

Anja Van De Stolpe

Roland Cornelis Martinus Vulders

Freek Van Hemert

Geeske Christina Dam-De Veen

Robby Petrus Cornelis Van Dreumel

Rudolf M J N Lamerichs

A method for stratification of a patient for assessing suitability of a therapy for the patient suffering from an ER (estrogen receptor) and/or PR (progesterone receptor) positive and HER2 (human epidermal growth factor receptor 2) negative cancer, the therapy being directed towards a signaling pathway, includes: (i) determining an activation status of an ER and/or PR signaling pathway by applying a Proximity Ligation Assay to detect in a tissue sample of the patient a presence of at least one member of the ER family, the at least one member being part of a transcription factor complex, and at least one protein selected from a group consisting of TAFs (TATA-binding protein associated factor), TBP (TATA-box binding protein), POLII (RNA polymerase II), TFII (transcription factor H), p300, CREB (cyclic-AMP response element-binding protein), and CBP (CREB binding protein), wherein the at least one protein is part of the same transcription factor complex.

Stratification of patients for assessing the suitability of a therapy

The present invention relates to an apparatus () for determining cellular composition in one or more tissue sample microscopic images. It is described to provide () first image data of at least one tissue sample. The first image data relates to a non-specific staining of a tissue sample of the at least one tissue sample. Second image data of the at least one tissue sample is provided (). The second image data relates to specific staining of a tissue sample of the at least one tissue sample. Either (i) the tissue sample that has undergone specific staining is the same as the tissue sample that has undergone non-specific staining, or (ii) the tissue sample that has undergone specific staining is different to the tissue sample that has undergone non-specific staining. A non-specific cellular composition cell density map is determined () on the basis of the first image data. A specific cellular composition cell density map is determined () on the basis of the second image data. Information regarding cellular composition in the at least one tissue sample is determined () on the basis of the non-specific cell density map and the specific cell density map.

Apparatus for determining cellular composition information in one or more tissue samples

The present application relates to a device for the electroporation of bacterial cells in or on a surface of the Stratum corneum layer of a person's skin. It comprises electrodes positionable in the vicinity of said surface; a generator to supply a voltage to the electrodes to generate an electrical field having a strength in the order of 25 to 35 KV/cm at said surface of the Stratum corneum layer to inactivate bacterial cells in or on said surface. The electrodes are configured so that the strength of the electrical field reduces as a function of the depth of penetration into the skin from 25 to 35 KV/cm at said surface to 3 KV/cm or less at a depth of penetration that does not exceed 15 microns. The electrical field generated by the device will have sufficient strength to inactivate bacteria cells present on the stratum corneum while at the same time this electrical field is not strong enough to appreciable effect living skin cells in the epidermis below the stratum corneum or at the interface between the stratum corneum and the epidermis.

Device for inactivating bacteria on the surface of the stratum corneum layer of skin

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Eindhoven, Netherlands

David Halter