Purdue Research Foundation

Other

David H Thompson

Seok-Hee Hyun

Aditya Kulkarni

Christopher Collins

Yawo Mondjinou

Kyle J Wright

Wei Deng

Ruben Claudio Aguilar

Scott Charles Bolton

Timothy L Ratliff

Scott Crist

Christopher Benjamin

Robert Graham Cooks

Philip Stewart Low

Kinam Park

Cagri Abdullah Savran

Gil U Lee

Chun-Li Chang

Christina Ramires Ferreira

Osman Basaran

Ghanashyam S Acharya

Derek Doorneweerd

Walter A Henne

Christine A Hrycyna

Zinia Jaman

David Brownholland

Igal Szleifer

Jeremy A Boomer

Robert Haynes

Valerie C Anderson

Methods of using grids in connection with suitable microscopy techniques, such as for determining the structure of target compounds including proteins, are disclosed. Said grid comprising a coating modified with one or more capture agents and a deactivating agent.

Grid coatings for capture of proteins and other compounds

Various embodiments of the present invention are directed to polyrotaxanes comprising a poloxamer core and at least one cyclodextrin and methods for treating Niemann-Pick type C (NPC) and imaging (e.g., MRI) using the polyrotaxanes of the various embodiments of the present invention.

Polyrotaxanes bearing mixed cyclodextrin species and uses thereof

Grids comprising a coating modified with one or more capture agents and a deactivating agent are disclosed. Methods of using such grids in connection with suitable microscopy techniques, such as for determining the structure of target compounds including proteins, are also disclosed.

Disclosed herein is a continuous manufacturing process for lomustine that has a short residence time and 63 percent yield. Major advantages of this process are that the total production cost for lomustine is lower, the product is higher quality, and the manufacturing operation is safer for production personnel.

On-demand rapid synthesis of lomustine under continuous flow conditions

Various embodiments of the present invention are directed to polyrotaxanes comprising a poloxamer core and at least one cyclodextrin and methods for treating Niemann-Pick type C (NPC) and imaging (e.g., MRI) using the polyrotaxanes various embodiments of the present invention.

Therapeutic and imaging compositions and uses thereof

A polyrotaxane containing an affinity binding group has been designed and prepared. The polyrotaxane of the invention can be used for characterization and determination of the three-dimensional structures of biological molecules, such as proteins.

Polyrotaxanes and uses thereof

Composition and methods are disclosed for utilizing microaggregation of FAP-containing complexes to promote their fast internalization. This approach allows the uptake of cytotoxic cargo coupled to either FAP-Antibodies or FAP-liposome complexes by tumor bladder cells. Importantly, this approach is efficient even under serum-free conditions such as the ones found in the lumen of the bladder.

Treating bladder tumor cells using fibronectin attachment protein as a target

The invention relates to nucleic acid complexes, methods of preparation thereof, and uses thereof for delivering a nucleic acid into a cell.

Nucleic acid complexes

Composition and methods are disclosed for utilizing micro-aggregation of FAP-containing complexes to promote their fast internalization. This approach allows the uptake of cytotoxic cargo coupled to either FAP-Antibodies or FAP-liposome complexes by tumor bladder cells. Importantly, this approach is efficient even under serum-free conditions such as the ones found in the lumen of the bladder.

Systems and methods for rapid and ultrasensitive detection of target biomolecules in a sample are presented. The detection of biomolecules is achieved through a synergistic use of immunoseparation and diffractometry, and the formation of antibody-biomolecule-ligand sandwich complexes that form diffraction gratings. Characteristic diffraction patterns are then produced upon illumination of the diffraction gratings with light. The diffraction patterns can be used to detect very low amounts of biomolecules present in the sample.

Ultrasensitive detection of biomolecules using immunoseparation and diffractometry

Bioanalytical device that includes a biofunctional component and an optional sensor component. The device includes arrays of addressable, durable, asymmetric biofunctional membranes containing protein transducers capable of unidirectional transport of analytes. Suitable protein transducers include members of the ATP-binding cassette family, such as P-glycoprotein.

Device and bioanalytical method utilizing asymmetric biofunctionalized membrane

A novel amphiphilic lipid compound having a cleavable, vinyl ether linked hydrophilic headgroup is described. Also described are liposomes containing the vinyl ether lipid compound, which may be triggered to release their contents and/or permeablize or fuse with target lipid membranes. The cleavable vinyl ether linkage allows the hydrophilic headgroup to be dissociated from the hydrophobic tailgroup(s) of the lipid compound to facilitate phase transitions in the lipid bilayer.

Vinyl ether lipids with cleavable hydrophlic headgroups

A triggered release liposomal delivery system is disclosed that selectively releases its contents in response to illumination or reduction in pH. The liposomes contain an amphipathic lipid, such as a phospholipid, having two chains derived from fatty acid that allow the lipid to pack into a bilayer structure. One or both of the alkyl chains contains a vinyl ether functionality that is cleaved by reactive oxygen species (ROS) or acid. A photosensitizer is incorporated into the liposomal cavity or membrane, and produces ROS or acid when illuminated to cleave the vinyl ether functionality and disrupt the liposomal membrane to release the vesicle contents. The lipid is preferably a plasmalogen, for example ##STR1## wherein R.sub.1 and R.sub.2 are each long chain hydrocarbons containing 12-24 carbons.

Growing community of inventors

West Lafayette, IN, United States of America

David H Thompson