University of Michigan

University of Minnesota

Colorado State University Research Foundation

Allegheny-singer Research Institute

University of California

Rensselaer Polytechnic Institute

Inha-industry Partnership Institute

Darpa

Asociacion Instituto Nacional De Biodiversidad

David H Sherman

Zachary Q Beck

Ashootosh Tripathi

Robert M Williams

Amy E Fraley

Michael Marie Kaufman-Schofield

Garth David Ehrlich

Nathan Magarvey

Yousong Ding

Yongquan Xue

Benjamin Janto

Christopher M Rath

Sunit Jain

Gregory Dick

Jungbae Kim

Jonathan S Dordick

Douglas S Clark

Michael C Flickinger

Aravind Srinivasan

Kyu B Han

Chuanwu Xi

Eung-Soo Kim

Janet L Smith

Zixin Deng

Liangcai Gu

Jianfeng Wu

Paul R August

Mark D Williams

Linquan Bai

Wolfgang Seufert

Giselle Tamayo

Jennifer J Schmidt

Pam Schultz

Shengying Li

Mustafa Varoglu

Krithika Anand Srinivasan

Yingqing Mao

Paul Sheldon

Pamela J Schultz

Min He

Sung Ryeol Park

Hung-Wen Liu

Lishan Zhao

Shuangjun Lin

Dekun Kong

Mi-Jin Lee

Fengan Yu

Jennifer Gehret

Kathleen L Collins

Andrew W Robertson

Mark M Painter

The disclosure provides biocatalysts that halogenate complex chemical compounds in specific and predictable ways. Also disclosed are halogenated complex organic compounds. The disclosure further provides methods for the halogenation of complex chemical compounds and methods of inhibiting the contraction of smooth muscle in mammals.

Halogenation of complex organic compounds

The disclosure provides cryptophycin intermediates, cryptophycin analogs, and cryptophycin chimeric molecules useful in treating cancer, as well as methods of producing these compounds and methods of treating cancer.

Biocatalytic synthesis of cryptophycin anticancer agents

Provided herein are biofilm inhibitors obtained from marine microbial derived natural product extracts from, resulting in biofilm inhibitors, cahuitamycins. Also provided are mutant, methods of inhibiting biofilm formation, methods of producing, or increasing the production of, cahuitamycins, methods for synthesizing cahuitamycins, and methods of purifying cahuitamycins.

Cahuitamycins and methods of making and using the same

The present disclosure is directed to the biosynthetic pathway for a nonribosomal peptide synthetase (NRPS) derived drug and analogs thereof. The invention provides polynucleotide sequences useful for heterologous expression in a convenient microbial host for the synthesis of the NRPS-derived drug, the polypeptides encoded by such polynucleotides, expression vectors comprising the polynucleotides, host cells comprising the polynucleotides or expression vectors, and kits comprising a host cell. Also provided is a method for the production of ET-743, the NRPS-derived drug.

Nonribosomal peptide synthetases

Provided herein are compositions of compounds of formula (I), methods of inhibiting a bacterial infection by contacting a cell with a composition comprising a compound of formula (I), and methods of isolating compounds of formula (I) from an extract of

Baulamycins, compositions and methods of use thereof

The biosynthesis of fungal bicyclo[2.2.2]diazaoctane indole alkaloids with a wide spectrum of biological activities have attracted increasing interest. Their intriguing mode of assembly has long been proposed to feature a non-ribosomal peptide synthetase, a presumed intramolecular Diels-Alderase, a variant number of prenyltransferases, and a series of oxidases responsible for the diverse tailoring modifications of their cyclodipeptide-based structural core. Until recently, the details of these biosynthetic pathways have remained largely unknown due to lack of information on the fungal derived biosynthetic gene clusters. Herein, we report a comparative analysis of four natural product metabolic systems of a select group of bicyclo[2.2.2]diazaoctane indole alkaloids including (+)/(&#x2212;)-notoamide, paraherquamide and malbrancheamide, in which we propose an enzyme for each step in the biosynthetic pathway based on deep annotation and on-going biochemical studies.

Biosynthetic systems producing fungal indole alkaloids

The present invention is directed to the biosynthetic pathway for a nonribosomal peptide synthetase (NRPS) derived drug and analogs thereof. The invention also discloses polynucleotide sequences useful for heterologous expression in a convenient microbial host for the synthesis of the NRPS derived drug.

Biosynthetic pathway for heterologous expression of a nonribosomal peptide synthetase drug and analogs

The present invention relates to a novel polyene compound similar to nystatin, a method for preparing the same, and an antifungal drug comprising the novel polyene compound as an active ingredient. Compared to nystatin comprising one sugar, NNP, which is the polyene compound similar to nystatin, displays 300 times higher solubility and ten times lower cytotoxicity while maintaining antifungal activity, and thus can be useful in developing a novel polyene antifungal agent having improved solubility and less toxicity in terms of pharmacokinetics.

Polyene compound, method for preparing the same, and antifungal drug comprising novel polyene compound as active ingredient

The present disclosure is directed to the biosynthetic pathway for a nonribosomal peptide synthetase (NRPS)-derived drug and analogs thereof. The invention provides polynucleotide sequences useful for heterologous expression in a convenient microbial host for the synthesis of the NRPS-derived drug, the polypeptides encoded by such polynucleotides, expression vectors comprising the polynucleotides, host cells comprising the polynucleotides or expression vectors, and kits comprising a host cell. Also provided is a method for the production of ET-743, the NRPS-derived drug.

Disclosed herein are methods of preparing alkenes from beta-hydroxy or beta-sulfate carboxylic acid or carboxylic acid derivatives using thioesterase and optionally a sulfotransferase.

Method for enzymatic production of decarboxylated polyketides and fatty acids

The present invention provides polypeptides involved in cryptophycin biosynthesis and the nucleic acid molecules that encode such polypeptides. The nucleic acid molecules and polypeptides of the invention or variants thereof can be used in the methods of the invention to produce cryptophycins.

Nucleic acids and polypeptides involved in the production of cryptophycin

A method of preparing macrocycles using solid support chemistry and thioesterases is disclosed. Also disclosed are novel macrocycles.

Macrocyclization of compounds from solid support using thioesterases

Growing community of inventors

Ann Arbor, MI, United States of America

David H Sherman