Cepheid Corporation

Corixa Corporation

David H Persing

Ronald G Chang

David A Johnson

David Swenson

Alan Wortman

Joseph H Smith

Rong Wang

Jory R Baldridge

J Terry Ulrich

Richard Thomas Crane

Michael J Lacy

Alexander A Gall

Raymond L Houghton

Sergey G Lokhov

Paul R Sleath

Olivier Delfour

Ekaterina V Viazovkina

Vera Baraznenok

Irina Shishkina

Bernard Michot

David Vilanova

William E Murray

Cecilia Svanholm Barrie

Gary T Elliot

Oliver Delfour

The present disclosure relates to aptamers for temperature-dependent reversible inhibition of thermostable polymerase activity in order to improve sensitivity and specificity of various reactions and assays involving hot start polynucleotide synthesis. Methods for use of the aptamers and related compositions and kits are also provided.

Thermostable polymerase inhibitor compositions and methods

The invention provides methods and apparatus for carrying out multiple amplification reactions in a single reaction chamber by successive cycles of loading reaction mixture, amplifying, and removing spent reaction mixture in a fluidly closed reaction system. In particular, the present invention allows amplification of a plurality of target polynucleotides from a single sample by carrying out under closed-loop control successive amplifications of different target polynucleotides from different portions of the sample.

Methods and apparatus for sequential amplification reactions

Compositions and methods for detecting(CT) and(NG) are provided. The present invention also provides methods and compositions for screening for infection/inflammation based on genomic copy number. Described herein is a method that entails assaying a sample obtained from the urogenital tract of the mammal for an indicator of genomic copy number, wherein a genomic copy number level that is higher than a control genomic copy number level is indicative of the presence of infection or inflammation of the urogenital tract. Also described in a kit of the invention that includes a primer and/or probe for detecting or sequencing an indicator of genomic copy number, wherein the indicator of genomic copy number comprises a nucleic acid sequence that is expected to be present in the genome of the mammal in one or two copies; and a primer and/or probe for detecting or sequencing a nucleic acid sequence that is indicative of a pathogen that infects the urogenital tract or a miRNA correlated with inflammation.

Methods of detecting chlamydia and gonorrhea and of screening for infection/inflammation based on genomic copy number

Methods of detecting cervical dysplasia, such as cervical dysplasia likely to progress to carcinoma in a sample of human cervical cells, are provided. Methods of detecting changes in expression of one or more microRNAs or mRNAs associated with cervical dysplasia or cervical cancer are also provided. Compositions and kits are also provided.

Methods of detecting cervical cancer

Methods of detecting sepsis in a sample from a patient are provided. Methods of detecting changes in expression of one or more microRNAs associated with sepsis are also provided. Compositions and kits are also provided.

Methods of detecting sepsis

Methods and compositions for treating or ameliorating diseases and other conditions, such as infectious diseases, autoimmune diseases and allergies are provided. The methods employ mono- and disaccharide-based compounds for selectively stimulating immune responses in animals and plants.

Prophylactic and therapeutic treatment of infectious and other diseases with mono- and disaccharide-based compounds

Compounds that are adjuvants and immunoeffectors are described and claimed. The compounds augment antibody production in immunized animals as well as stimulate cytokine production and activate macrophages. Compositions and methods for using the compounds as adjuvants and immunoeffectors are also disclosed.

Certain aminoalkyl glucosaminide phosphate compounds and their use

Compositions and methods for the therapy and diagnosis of cancer, particularly breast cancer, are disclosed. Illustrative compositions comprise one or more breast tumor polypeptides, immunogenic portions thereof, polynucleotides that encode such polypeptides, antigen presenting cell that expresses such polypeptides, and T cells that are specific for cells expressing such polypeptides. The disclosed compositions are useful, for example, in the diagnosis, prevention and/or treatment of diseases, particularly breast cancer.

Compositions and methods for the therapy and diagnosis of breast cancer

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San Martin, CA, United States of America

David H Persing