Talis Biomedical Corporation

Bio-Rad Laboratories, Inc.

David Glade

Thomas Henry Cauley, Iii

John Dixon

Michael John McAdams

Hédia Maamar

David Alexander Rolfe

Sayeed Andeshmand

Dzam-Si Jesse Ng

David P Stumbo

Dmitri Simonian

Joshua T Oen

Denis Pristinski

John Dzenitis

George Carman

Steve Hobbs

Anthony Makarewicz

Anthony Joseph Makarewicz, Jr

Methods and systems are provided for point-of-care nucleic acid amplification and detection. One embodiment of the point-of-care molecular diagnostic system includes a cartridge and an instrument. The cartridge can accept a biological sample, such as a urine or blood sample. The cartridge, which can comprise one or more of a loading module, lysis module, purification module and amplification module, is inserted into the instrument which acts upon the cartridge to facilitate various sample processing steps that occur in order to perform a molecular diagnostic test.

Diagnostic system

Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.

System and method for droplet detection

Growing community of inventors

San Ramon, CA, United States of America

David Glade