South Dakota State University

David Francis

Weiping Zhang

Vaccines and methods for making and using the same. An example vaccine may be a vaccine against enterotoxigenic. The vaccine may include anstrain. Thestrain may produce K88 fimbria and a fusion protein including a mutant LT enterotoxin linked with a STb enterotoxin. An example method for producing a vaccine for porcine post-weaning diarrhea may include providing a first strain of. The strain may include the eltAB gene and the estB gene. The method may also include amplifying the eltAB gene, mutating the eltAB gene, generating a genetic fusion of the mutant eltAB gene with the estB gene, and transforming a second strain ofwith the genetic fusion.

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Brookings, SD, United States of America

David Francis