Ohio State Innovation Foundation

The United States of America, As Represented by the Secretary, Department of Health and Human Services

Invitrogen Corporation

David F Nellis

Jianwei Zhu

David Webster Wood

Buyong Ma

Dennis F Michiel

Stephanie Nilsen

Disclosed are compositions comprising an engineered intein designed such that the self-cleaving activity of the intein can be modulated by a zinc-binding motif as well as methods and systems for making and using the compositions.

Reversible regulation of intein activity through engineered new zinc binding domain

Compositions related to controllable intervening protein sequences (CIPS) comprising reversible zinc-binding motifs and inteins

The invention provides IL-15 amino acid sequences with amino acid substitutions that reduce or eliminate deamidation of IL-15 and degradation by-products. The invention also provides DNA sequences that encode the substituted amino acid sequences, a pharmaceutical composition comprising the substituted IL-15 amino acid sequence and a pharmaceutically acceptable carrier, and a method of treating a condition in a mammalian host comprising administering to the host the substituted IL-15 amino acid sequence or the pharmaceutical composition including the substituted IL-15 amino acid sequence in an amount effective to treat the condition in the host.

Substituted IL-15 polypeptides

A system, method and computer program product is provided for processing images of an electrophoretic separation medium to determine the unit activity of an enzyme. A test aliquot, comprising a macromolecule (such as, DNA, RNA, protein, peptide or the like) and diluted enzyme concentration, is distributed in the separation medium. The enzyme concentration acts as a catalysis to cleave the macromolecule into distinct fragments during electrophoresis. A set of intensity data profiles are produced from images of the fragments. The profiles are stacked and vertically aligned to designate and assign the fragments to their respective lanes. A group of partial bands and final bands are selected from the fragments. Peak integrations are implemented to measure the intensity of the partial and final bands. A series of intensity ratios are computed from the peak integrations. The intensity ratios are normalized and plotted to produce a trend. A threshold crossing value is derived from the effective dilution factor corresponding to a point at which the trend crosses a threshold crossing level. The threshold crossing value is used to determine a calibration factor. The calibration factor used to determine a reported unit call.

Method for measuring unit activity of an enzyme

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Frederick, MD, United States of America

David F Nellis