Arcturus Engineering, Inc.

Spectra-physics Gmbh

Arcturus Bioscience, Inc.

Life Technologies Corporation

Spectra Physics Lasers, Inc.

Molecular Devices Corporation

Spectra Physics Laser Diode Systems

Arturus Bioscience, Inc.

David F Head

Thomas Michael Baer

Christopher E Todd

Mark A Enright

Robert H Reamey

John Toeppen

Bruce J Richardson

Keith Moravick

Derrick A Richardson

Phillip Gooding

Gregory J Kintz

James D Kafka

Lee R Carlson

Alan B Petersen

John L Cole

Steven T Kunitake

Joseph B Lastovka

Robert C Rempel

Hala Al-Shawany

Barbara J Wessen-Baer

Kelly A Cox

Ali Firouzi

David Ching

Ann Bennett Lossing

Sherrie L Ransom

Keith E Moravick

The present invention generally discloses an extraction system that provides a locale for fluid processing and extraction on a post-microcapture transfer film. The extraction system includes a transfer film carrier and an extraction device forming a reservoir. The extraction system selectively excludes regions of the transfer film from the reservoir to advantageously reduce contamination due to matter adhered to the transfer film by non-specific transfer.

Laser capture microdissection (LCM) extraction device and device carrier, and method for post-LCM fluid processing

Systems and methods for acquiring laser capture microdissection samples are disclosed. A scattering media is located within a beam path defined by the laser capture microdissection microscope and the sample is imaged through the scattering media. A transfer film carrier includes a substrate surface and a laser capture microdissection transfer film coupled to the substrate surface of the transfer film carrier. The scattering media is optically coupled to a laser capture microdissection transfer film being located in or attached to the laser capture microdissection transfer film or transfer film carrier. The systems and methods provide improved sample imaging and facilitate quick and accurate laser capture microdissection while simultaneously minimizing contamination.

Consumable for laser capture microdissection

Systems and methods for acquiring laser capture microdissection samples are disclosed. An integral portion of a biological reaction vessel includes a transfer film carrier having a substrate surface; and a laser capture microdissection transfer film coupled to the substrate surface of the transfer film carrier. The systems and methods facilitate quick and accurate laser capture microdissection while simultaneously minimizing contamination.

Systems and methods for laser capture microdissection are disclosed. An inverted microscope includes a translation stage joystick subsystem. The systems and methods provide the advantages of increased speed and much lower rates of contamination.

Laser capture microdissection translation stage joystick

Systems and methods for laser capture microdissection are disclosed. An inverted microscope includes a vacuum chuck subsystem that is adapted to hold a microscope slide down during the process of laser capture microdissection. The systems and methods provide the advantages of increased speed and much lower rates of contamination.

Laser capture microdissection vacuum hold-down

The present invention generally provides an improved transfer film having multiple layers for laser micro-capture of a sample. The transfer film for laser micro-capture includes distinct layers for expansion and adhesion in order to optimize the performance of the transfer film. A transfer film including a spring-back layer is also disclosed.

Transfer film for laser microcapture

Systems and methods for laser capture microdissection are disclosed. An inverted microscope includes an illumination/laser beam delivery system that is adapted to both illuminate a sample and provide energy for laser capture microdissection of the sample. The systems and methods provide the advantages of increased speed and much lower rates of contamination.

Laser capture microdissection optical system

Systems and methods are described for barriers on laser capture microdissection samples. A method of processing a biological sample for laser capture microdissection, includes: providing the biological sample; and applying a substance to the biological sample so as to provide a barrier between the biological sample and a surrounding environment. The systems and methods provide advantages because non-specific pick-up is reduced, visualization is improved, sample degradation is reduced, and contamination is reduced.

Processing technology for LCM samples

A thermoplastic film for LCM tissue transfer is thermally coupled to a broadband energy-absorbing material. The broadband energy-absorbing material may either be introduced into the film composition as a dopant or may be in thermal contact with the film.

Broadband absorbing film for laser capture microdissection

Systems and methods for laser capture microdissection are disclosed. A method of laser capture microdissection includes providing a sample that is to undergo laser capture microdissection; positioning said sample within an optical axis of a laser capture microdissection instrument; providing a transfer film carrier having a substrate surface and a laser capture microdissection transfer film coupled to said substrate surface; placing said laser capture microdissection transfer film in juxtaposition with said sample with a pressure sufficient to allow laser capture microdissection transfer of a portion of said sample to said laser capture microdissection transfer film, without forcing nonspecific transfer of a remainder of said sample to said laser capture microdisection film; and then transferring a portion of said sample to said laser capture microdissection transfer film, without forcing nonspecific transfer of a remainder of said sample to said laser capture microdissection transfer film. The systems and methods provide the advantages of increased speed and much lower rates of contamination.

Laser capture microdissection method and apparatus

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Los Gatos, CA, United States of America

David F Head