Sri International

University of California

David E Huber

Roy D Kornbluh

Qibing Pei

Maneesh Yadav

Kevin Luebke

Ziyang Zhang

Rujun Ma

Keith Laderoute

Kathryn G Todd

Matthew D Puster

Embodiments in accordance with the present disclosure are directed to methods and apparatuses used for evaluating a protein in a sample using proteolysis and translocation. An example method includes isolating a single protein from a sample in a chamber having at least a first well and a second well separated from the first well by a membrane with a nanopore, the nanopore providing fluidic communication between the first and second wells. The single protein is cleaved into a plurality of peptide fragments via exposure to a protease in the first well, and translocated through the nanopore by applying an electric potential to the nanopore in the chamber after cleavage of the single protein by the protease. The method further includes detecting events indicative of the translocations of the plurality of peptide fragments through the nanopore and to the second well.

Apparatuses and methods involving protein exploration through proteolysis and nanopore translocation

A solid-state heat transporting device including a heat transporting element whose uniformity of contact with one or multiple surfaces is controllable so that various amounts of heat may be transported to and from the one or multiple surfaces. The heat transporting element uses the electrocaloric effect to absorb and release the heat and the uniformity of contact is controlled using an electrostatic effect which may change the shape of the heat transporting element. In one embodiment, the heat transporting element is an electrostatically actuated P(VDF-TrFE-CFE) polymer stack achieving a high specific cooling power of 2.8 W/g and a COP of 13 (the highest reported coefficient of performance to date) when used as a cooling device.

Electrocaloric cooling with electrostatic actuation

Devices, systems and methods for the parallel detection of a set of distinct nucleic acid sequences use multiple sequence amplification and simultaneous hybridization readout. An automated nucleic acid analysis system comprises in microfluidic connection sample lysis, purification, PCR and detection modules configured to detect in parallel distinct nucleic acid sequences via multiple sequence amplification and simultaneous microarray hybridization readout. High performance microfluidic electroactive polymer (&#x3bc;EAP) actuators comprising a dead-end fluid chamber in which a surface of the chamber is an electrode covered with an EAP layer of dielectric elastomer are configured for particle sorting.

Portable nucleic acid analysis system and high-performance microfluidic electroactive polymer actuators

Growing community of inventors

Menlo Park, CA, United States of America

David E Huber