Danisco US Inc.

David E Estell

Andrew Shaw

Sandra W Ramer

William Albert Cuevas

Sang-Kyu Lee

Amr R Toppozada

Scott Douglas Power

Michael Jay Pepsin

Louise Wallace

Carol A Requadt

Regina Chin

Sura H Hadi

Alpha-amylases from(AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.

[object Object]

Described are variants of a parent &#x3b1;-amylase that exhibits an alteration in at least one of the following properties relative to said parent &#x3b1;-amylase: specific activity, substrate specificity, substrate binding, substrate cleavage, thermal stability, pH-dependent activity, pH-dependent stability, oxidative stability, Ca2+ dependency, pI, and wash performance. The variants are suitable for starch conversion, ethanol production, laundry washing, dish washing, hard surface cleaning, textile desizing, and/or sweetener production.

Growing community of inventors

San Francisco, CA, United States of America

David E Estell