Chemimage Corporation

Eastman-kodak Company

Other

United States of America, As Represented by the Secretary of Agriculture

David D Tuschel

Thomas Voigt

Patrick J Treado

John Maier

Chenhui Wang

Wesley H Hutchison

Arjun S Bangalore

Seshadri Jagannathan

David John Nelson

Ramesh Jagannathan

Joseph E Demuth

Xinghua Wang

Dilip K Chatterjee

Matthew P Nelson

James Michael Chwalek

Gustavo R Paz-Pujalt

Sridhar Sadasivan

Thomas N Blanton

Glen C Irvin, Jr

Suresh Sunderrajan

Moon S Kim

Kuanglin Chao

Jingyun Zhang

Alan M Lefcourt

Byron R Sever

Oksana Klueva

Oksana Olkhovyk

Charles R Moon

Myles P Berkman

Yongliang Liu

William C Lenhart

System and method for determining the presence of a contaminant in a sample using Raman spectroscopic data. The sample may be food or feed and the contaminant may be melamine. The sample is illuminated with substantially monochromatic light to produce Raman scattered photons. The Raman scattered photons are collected to generate Raman spectroscopic data. The Raman spectroscopic data may comprise at least one of a Raman spectrum and a spatially accurate wavelength resolved Raman image. The Raman spectroscopic data is analyzed to determine the presence or absence of a contaminant in a sample. The concentration of the contaminant in the sample can also be determined by using a ratio algorithm.

Systems and methods for detecting contaminants in a sample

A method and apparatus of obtaining a spectral image of a plurality of predetermined chemical species. A sample is illuminated to produce photons. These photons are collected to produce a plurality of images for each predetermined chemical species, wherein each image comprises a frame consisting of a plurality of pixels. A wavelength range is identified wherein a chemical species exhibits a unique absorption of radiation. Pixels are identified that do not comprise the chemical species. The steps may be repeated for a plurality of chemical species. If more than one chemical species is present, the contribution of each in a pixel is separated and separate spectral images of each species is composed.

Method and apparatus for interactive hyperspectral image subtraction

The disclosure relates generally to methods and apparatus for protecting against counterfeit products and for methods and apparatus for providing counterfeiting protection for an object. In one embodiment, the method includes the steps of: (a) accessing an object including an identifying mark having a first portion and a second portion wherein said second portion includes an encrypted feature; (b) reading the first portion to thereby obtain a first set of information; (c) transmitting the first set of information to a remote location; (d) receiving from the remote location a second set of information; and (e) reading the second portion using the second set of information to thereby determine the authenticity of the object.

Method and apparatus for counterfeiting protection

The disclosure relates to systems and method for chemical imaging of microarrays. In one embodiment, the disclosure relates to a system for simultaneous spectral imaging of a plurality of samples arranged on an array. The system includes an illumination source for providing illuminating photons to said plurality of samples, the illuminating photons interacting with each of the plurality of samples to emit interacted photons; an array for receiving said plurality of samples, the array having an external dimension such that the samples are within a simultaneous field of view of the optical device; an optical device for collecting the interacted photons and directing the photons to an imaging device, the imaging device simultaneously forming a plurality of images corresponding to each of the plurality of samples.

System and method for chemical imaging of microarrays

A pulse photobleaching methodology wherein a monochromatic illumination (e.g., laser illumination) having a higher power intensity (photobleaching power) just below the photodamage threshold of a luminescent sample is initially used to significantly attenuate sample luminescence without photothermally destroying the sample material. Thereafter, the laser power density may be reduced to a significantly lower level (analytical power level) to carry out spectroscopic measurements (e.g., collection of Raman scattered photons) on the sample. In one embodiment, the laser illumination wavelength remains the same despite changes in laser power intensity. Some figures-of-merit may be computed from optical measurements made at the analytical power level to guide the photobleaching process. Sample-dependent combinations of laser power density and short exposure times may be obtained to significantly expedite photobleaching to assist in collection of sample spectral data in the field without a long wait. Portable spectroscopy systems employing pulse photobleaching may be devised for expeditious collection of spectral data from luminescent samples in the field.

Photobleaching by high power pulses

A system and method for collecting Raman data sets without the 'contaminating' effect of luminescence emitted photons. Using a frame transfer CCD for time resolved data collection, Raman imaging may be performed without photobleaching the sample. The system may include a light source, a frame transfer CCD, an optical lens and at least one controller. The light source illuminates the sample with a plurality of photons to generate scattered photons from the sample. The frame transfer CCD has an image array and a storage array. The optical lens collects scattered photons and directs the scattered photons to the image array. The controller transfers a Raman data set representative of the scattered photons from the image array to the storage array. The frame transfer CCD may be configured so as the image array integrates the scattered photons during a Raman integration time and the controller transfers the Raman data set from the image array to storage array during a parallel transfer time. The sum of the Raman integration time and the parallel transfer time is less than the minimum time it takes to trigger luminescence from the sample.

System and method to perform raman imaging without luminescence

A single detector based spectroscopy system using FAST (fiber array spectral translator) fibers and two excitation sources in conjunction with a holographic spectrum analyzer (HSA) to obtain simultaneous and selective display of spectroscopic regions of interest. A sample can be illuminated with different laser excitation wavelengths and resulting multiple spectra can be comparatively observed on a single display screen for more fruitful analysis of sample spectral responses (and, hence, sample chemical or physical properties) under different excitations. The HSA may be configured to focus on user-selected spectral regions of interest from different such spectra and a single CCD detector may be configured to collect spectral data from all selected spectral regions of interest in corresponding portions of the CCD pixel array, thereby allowing subsequent simultaneous display of such selected spectral regions of interest. The HSA may also allow simultaneous collection and display of portions of a single spectrum from a single excitation wavelength. A user can perform better comparative analysis when spectral regions of interest are juxtaposed with each other on a single electronic display.

Single detector based dual excitation wavelength spectra display

The present disclosure describes methods and apparatus to produce a streaming image of a sample during a time period when an attribute of the sample is changing. The streaming image can be viewed in such a manner so as to be able to follow a visible change in an attribute of the sample. The sample may be undergoing nucleation, aggregation, or chemical interaction. The present disclosure also describes methods and apparatus to determine a change in an attribute of a sample by detecting, analyzing, and comparing spectra of the sample taken at different times during the time period when the attribute of the sample is changing. The sample may be undergoing nucleation, aggregation, or chemical interaction.

Method and apparatus for producing a streaming Raman image of nucleation, aggregation, and chemical interaction

The invention relates to methods of dynamic chemical imaging, including methods of cellular imaging. The method comprises illuminating at least a portion of a cell with substantially monochromatic light and assessing Raman-shifted light scattered from the illuminated portion at a plurality of discrete times. The Raman-shifted light can be assessed at a plurality of Raman shift (RS) values at each of the discrete times, and the RS values can be selected to be characteristic of a pre-selected component at each of the discrete times. Multivariate analysis of Raman spectral features of the images thus obtained can yield the location and chemical identity of components in the field of view. This information can be combined or overlaid with other spectral data (e.g., a visible microscopic image) obtained from the field of view.

Dynamic imaging of biological cells and other subjects

An integrated system including one or more light sources, at least one processor, an optical lens, a two-dimensional tunable filter, one or more two-dimensional array of detection elements and instructions and a method using the integrated system. The system includes a plurality of modes: a Raman mode, an absorption mode, a luminescence mode, a crossed polarization mode, a crossed polarization absorption mode, bright field transmission or reflectance modes and a birefringence mode. The system includes instructions, executable by Sequential outputs from the two-dimensional array of detection elements is combined to generate a chemical image of the sample, wherein each of the sequential outputs from the first two-dimensional array of detection elements corresponds to spatially accurate wavelength-resolved images. The system is also used to detect dynamic changes in a sample over time by monitoring the sample using one or more of the modes.

System for obtaining images in bright field and crossed polarization modes and chemical images in raman, luminescence and absorption modes

A system and method for obtaining hyperspectral visible absorption images. The system includes a light source which illuminates a sample containing light absorbing material, a platform, an optical lens, a detector. The platform has a section for placement of the sample and a section devoid of sample. The transmitted photons produced by the sample and collected by the optical lens are separated into a plurality of wavelength bands using a filter or a fiber array spectral translator coupled to a spectrometer. The system includes a programmable code for operating in a sample mode or background mode and calculating an absorption image. In the background mode, the platform motion is controlled so the optical lens collects transmitted photons from the, portion of the platform devoid of sample to generate a background transmission image. In the sample mode, the platform motion is controlled so the optical lens collects transmitted photons from the sample to generate a sample transmission image. The sample transmission image and the background transmission image are used to generate a photon absorption image of the sample.

Hyperspectral visible absorption imaging of molecular probes and dyes in biomaterials

The present disclosure describes methods and systems that combine Raman spectroscopy performed in a manner that utilizes one or more of widefield illumination, simultaneous multipoint Raman spectral acquisition, and spectral unmixing for the purpose of high throughput polymorph screening. Features of this methodology include: (a) high throughput polymorph screening to reduce crystal orientation effects on Raman spectra; (b) in-well multi-polymorph screening using increased statistical sampling; and (c) multipoint spectral sampling to enable spectral unmixing.

System and method for fiber array spectral translator based polymorph screening

In one embodiment, the disclosure relates to a method for determining illumination parameters for a stained sample, the method may include providing a stained sample and obtaining an absorption band of the sample; obtaining an emission band of the sample and determining the illumination parameters for the sample as a function of the absorption band and the emission band of the sample.

Apparatus and method for chemical imaging of a biological sample with at least two spectral images of the sample obtained simultaneously

The disclosure generally relates to a multimode imaging apparatus for simultaneously obtaining multiple wavelength-discriminative spectral images of a sample. In one embodiment, the apparatus includes an image selector having a rotator assembly, the rotator assembly housing a first plurality of optical components, the image selector adapted to receive a illuminating photons having a first wavelength and direct the illuminating photons to the sample, the image selector adapted to receive illuminating photons interacted with the sample and selectively direct said interacted photons to one of a plurality of detection sources; a microscope turret housing a second plurality of components, the microscope turret adapted to receive illuminating photons having a second wavelength and direct the photons to the sample; the microscopic turret adapted to receive illuminating photons interacted with the sample and selectively direct said interacted photons to one of a plurality of detection sources; wherein substantially all of the interacted photons are selectively directed one of a plurality of detection sources to form multiple wavelength discriminative spectral images of the sample simultaneously.

Growing community of inventors

Rochester, NY, United States of America

David D Tuschel