Monsanto Technology LLC

Dekalb Genetics Corporation

David D Songstad

Michael T Mann

William L Petersen

Brenda A Lowe

Jyoti R Rout

David R Duncan

Anisha Akula

Wanggen Zhang

Joel B Wilks

Yuechun Wan

Tishu Cai

Shubha Subbarao

Richard A Yingling

Anita Otal

James R Larkins

T Michael Spencer

Nancy G Willetts

Hongyi Zhang

A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.

Methods for producing transgenic plants

Production of transformed plants is achieved by placing-inoculated explants directly onto a medium containing selective agents for suppressing growth ofand killing non-transformed explant cells.

The present invention relates to a novel transformation system for generating transformed corn plants. In particular, the invention relates to a rapid selection system at an elevated temperature that allows faster and more efficient transformation.

Methods for corn transformation

According to the invention, there is provided an inbred corn plant designated LIZL5. This invention thus relates to the plants, seeds and tissue cultures of the inbred corn plant LIZL5, and to methods for producing a corn plant produced by crossing the inbred corn plant LIZL5 with itself or with another corn plant, such as another inbred. This invention further relates to corn seeds and plants produced by crossing the inbred plant LIZL5 with another corn plant, such as another inbred, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of the inbred corn plant LIZL5, and also to the SSR and genetic isozyme typing profiles of inbred corn plant LIZL5. This invention further relates to methods of transforming the inbred LIZL5 and cells thereof, transformed plants produced by these methods, progeny transgenic plants, and seed derived therefrom.

Transformable inbred corn line LIZL5 and methods for use thereof

Growing community of inventors

Ballwin, MO, United States of America

David D Songstad