Yale University

Research, Incorporated

Other

Molecular Staging Inc.

David C Ward

Patricia Bray-Ward

Gil G Mor

Michael Speicher

Alexander A Waldrop, Iii

Pennina R Langer

Peter Lichter

Stephen Gwyn Ballard

Charles Meyer Radding

H William Prusoff

Tai-Shun Lin

Gurucharan Reddy

Efim Ilya Golub

Thomas Haaf

John T Wilson

Sherman M Weissman

Milton Waner

Michael J Lane

Louis M Fink

Gyanendra Kumar

Laura Manuelidis

John P Neenan

Yung-Chi Cheng

Thomas Cremer

Thomas Ried

Yupo Ma

Saul M Honigberg

Antonio Baldini

Stephen Gwynn Ballard

Jeffry J Leary

David J Brigati

Andrew A Welcher

Basil Rigas

The claimed invention describes methods to diagnose or aid in the diagnosis of cancer. The claimed methods are based on the identification of biomarkers which are particularly well suited to discriminate between cancer subjects and healthy subjects. These biomarkers were identified using a unique and novel screening method described herein. The biomarkers identified herein can also be used in the prognosis and monitoring of cancer. The invention comprises the use of leptin, prolactin, OPN and IGF-II for diagnosing, prognosis and monitoring of ovarian cancer.

Identification of cancer protein biomarkers using proteomic techniques

The present invention describes stem cells and progenitor cells derived from hemangiomas, including testing of angiogenic inhibitors using these cells. The invention as described is useful in providing a process to culture and propagate hemangioma stem cells and generate xenograft models to develop treatments for infantile hemangiomas and other types of vascular lesions.

Isolation and growth of stem cells from hemangiomas

Disclosed are compositions and a method for detection of nucleic acid sequences. The disclosed method uses a structured probe to distinguish between sequences. Structured probes are bifunctional molecules where one function is as a probe to a target nucleic acid sequence and the other function is as a detection sequence to facilitate detection of the probe. Structured probes include a detection sequence, sequence complementary to a target sequence, and sequences that form duplex regions (higher order structures). The duplex region is stable unless the probe hybridizes to the target sequence. The disclosed method involves hybridizing the structured probe to a target sequence and detecting the detection sequence on the structured probe. The detection sequence is available for detection only if the duplex region of the structured probe is disrupted. This links detection of the structured probe with the hybridization of the structured probe to the target sequence.

Nucleic acid detection using structured probes

Disclosed is a method and compositions for the sensitive detection of the amount and location of specific nucleic acid sequences. The method makes use of a branched oligomer, referred to as a lollipop oligomer, that has a tail portion, a right arm portion, and a left arm portion. These three components are joined at a common junction making a three-tailed structure. The two arms each end with sequences complementary to adjacent sequences in a target sequence. This allows the right and left arms to be ligated together when the oligomer is hybridized to the target sequence, thus topologically linking the oligomer to the target sequence. The tail portion can then be detected at the location of the target sequence.

Signal amplification with lollipop probes

The invention relates to a set of combinatorially labeled oligonucleotide probes each member thereof: (i) having a predetermined label distinguishable from the label of any other member of said set, and (ii) being capable of specifically hybridizing with a predetermined chromosome or nucleic acid molecule, and to the use of such molecules, alone or in concert with nucleic acid amplification methods.

Multiparametric fluorescence in situ hybridization

Compositions and methods are provided for identifying and diagnosing individuals at risk for a disease state associated with aberrant Rad41 foci.

Methods and compositions utilizing Rad51

This disclosure relates to a method of specifically decorating selected mammalian chromosomes and of detecting, identifying and and/or quantitating selected individual chromosomes, by means of chromosomal in situ suppression (CISS) hybridization. The method is useful in analyzing cells for the occurrence of chromosomes, chromosome fragments or chromosome aberrations.

Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization

Compositions and methods are provided for identifying agents which bind to or modulate Rad51.

A method is disclosed for determining the chromosomal identity of a sample of genomic DNA. The genomic DNA is amplified and labeled by polymerase chain reaction using primers substantially complementary to interspersed repetitive DNA sequences. The amplified and labeled genomic DNA fragments are then contacted with chromosomal DNA of known identity under conditions in which the chromosome-specific, but not the interspersed repetitive DNA sequences, of the amplified and labeled genomic DNA fragments are available for hybridization. Specific hybridization to chromosomal DNA of known identity determines the chromosomal identity of the sample of genomic DNA.

Amplification karyotyping

Growing community of inventors

Madison, CT, United States of America

David C Ward