Quanterix Corporation

Surface Logix, Inc.

Harvard College

Beth Israel Deaconess Medical Center, Inc.

Tecan Trading Ag

David C Duffy

David Fournier

David M Rissin

David R Walt

Cheuk Wai Kan

Olivier Schueller

Stewart Campbell

Gregory Kirk

Melina Amber Agosto

George M Whitesides

Linan Song

Enoch Kim

John Lawson

Olivier J A Schueller

Scott T Brittain

Michael Kagan

Mingwei Zhao

Todd Campbell

Andrew Rivnak

John A Rogers

Emanuele Ostuni

Rebecca Jane Jackman

Jeffrey D Randall

Dandan Shan

Evan Ferrell

Klavs F Jensen

Michael J Grogan

Kathleen M Vaeth

Gregory John Kellogg

Steven A Kates

Paul Sweetnam

Lei Chang

Ravi Kane

Ciaran Kelly

Nira Pollock

Ciaran Keely

The present invention relates to systems and methods for detecting analyte molecules or particles in a fluid sample and in some cases, determining a measure of the concentration of the molecules or particles in the fluid sample. Methods of the present invention may comprise immobilizing a plurality of analyte molecules or particles with respect to a plurality of capture objects. At least a portion of the plurality of capture objects may be spatially separated into a plurality of locations. A measure of the concentration of analyte molecules in a fluid sample may be determined, at least in part, on the number of reaction vessels comprising an analyte molecule immobilized with respect to a capture object. In some cases, the assay may additionally comprise steps including binding ligands, precursor labeling agents, and/or enzymatic components.

Ultra-sensitive detection of molecules or particles using beads or other capture objects

Described herein are systems and methods for extending the dynamic range of assay methods and systems used for determining the concentration of analyte molecules or particles in a fluid sample. In some embodiments, a method comprises spatially segregating a plurality of analyte molecules in a fluid sample into a plurality of locations. At least a portion of the locations may be addressed to determine the percentage of said locations containing at least one analyte molecule. Based at least in part on the percentage, a measure of the concentration of analyte molecules in the fluid sample may be determined using an analog, intensity-based detection/analysis method/system and/or a digital detection/analysis method/system. In some cases, the assay may comprise the use of a plurality of capture objects.

Methods and systems for extending dynamic range in assays for the detection of molecules or particles

Described are systems, devices, and methods which related to various aspects of assays for detecting and/or determining a measure of the concentration of analyte molecules or particles in a sample fluid. In some cases, the systems employ an assay consumable comprising a plurality of assay sites. The systems, devices, and/or methods, in some cases, are automated. In some cases, the systems, devices, and/or methods relate to inserting a plurality of beads into assay sites, sealing assay sites, imaging assay sites, or the like.

Systems, devices, and methods for ultra-sensitive detection of molecules or particles

Ultrasensitive and quantitative assays for detecting toxins of, which may involve digital ELISA, are provided. Also provided herein are differential detection assays that allow for distinguishing toxin B of highly virulentstrains from toxin B of less virulent strains.

Immunoassays for differential detection of clostridium difficile

Described herein are methods and systems for detecting DNA or RNA using single molecules array or other techniques. DNA or RNA from the sample may be fragmented and exposed to a first type of binding ligand and a second type of binding ligand that comprise nucleic acid sequences complimentary at least a portion of a sequence contained in the target DNA or RNA. At least a portion of the fragmented DNA or RNA associates with at least one of the first type of binding ligand and/or the second type of binding ligand, wherein the first type of binding ligand and second type of binding ligand comprises nucleic acid sequences complimentary to a different portions of a sequence contained in the DNA or RNA. A portion of the sample exposed to the binding ligands is analyzed to determine the number of fragmented DNA or RNA sequences.

Detection of DNA or RNA using single molecule arrays and other techniques

Described herein are systems and methods for the detection of and/or determination of a measure of the concentration of analyte molecules or particles in a fluid sample. In some cases, the systems and methods employ techniques to reduce or limit the negative effects associated with non-specific binding events. Certain methods of the present invention involve associating the analyte molecules at least a first type of binding ligand and at least a second type of binding ligand, and spatially segregating the analyte molecules into a plurality of locations on a surface. The presence of an analyte molecule at or in a location may be determined by determining the presence of both the first type of binding ligand and the second type of binding ligand.

Ultra-sensitive detection of molecules using dual detection methods

Growing community of inventors

Arlington, MA, United States of America

David C Duffy