Philip Morris Products S.a.

David Bovard

Antonin Sandoz

Karsta Luettich

Julia Hoeng

Shoaib Majeed

There is described a method of reducing or preventing the agglomeration of spheroids comprising the use of a cell culture device comprising: a cell culture chamber comprising a base and side walls extending from the base to enclose a volume of the cell culture chamber; an inlet in the base or side walls of the cell culture chamber adapted for fluid communication into the chamber; and an outlet in the base or side walls of the cell culture chamber adapted for fluid communication out of the chamber; wherein the base of the cell culture chamber comprises a discontinuous surface adapted to reduce or prevent the agglomeration of spheroids.

Cell culture device

There is disclosed a cell culture plate comprising at least two sequentially arranged wells and a channel adapted for fluid communication between the wells, wherein the channel is connected or coupled to a first pump via openings at each end of the channel, and wherein said first pump is operable to circulate fluid between the at least two wells. Devices and systems incorporating the cell culture plate and methods and uses employing same are also disclosed.

Cell culture plate, devices and methods for in vitro exposure

There is described an isolated 3-dimensional liver spheroid wherein said spheroid has: increased ATP content as compared to a 3-dimensional liver spheroid cultured in Complete William's E medium alone; the same or increased activity of cytochrome P450 1A1 and cytochrome P450 1B1 as compared to a 3-dimensional liver spheroid cultured in Complete William's E medium alone; and increased albumin secretion as compared to a 3-dimensional liver spheroid cultured in William's E medium alone.

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Erlach, Switzerland

David Bovard